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Adam M. Elkin, Sarah Robbins, Claudia S. Barros, Torsten Bossing
Neural stem cells (NSC) are multipotent, self-renewing cells that give rise to all neural cell types within the central nervous system. During adulthood, most NSCs exist in a quiescent state which can be reactivated in response to metabolic and signalling changes, allowing …
gb (code pays fourni par la source)
Laura Díaz, Jon Gil-Ranedo, Karolina J. Jaworek, Nsikan Nsek et autres
Cell commitment to tumourigenesis and the onset of uncontrolled growth are critical determinants in cancer development but the early events directing tumour initiating cell (TIC) fate remain unclear. We reveal a single-cell transcriptome profile of brain TICs transitioning into tumour growth using …
gb, pt (code pays fourni par la source)
Claudia S. Barros, Torsten Bossing
Here, we describe a protocol to remove single identified cells directly from Drosophila living brains and analyze their transcriptome. We detail the steps to harvest fluorescent cells using a capillary under epifluorescence and transmitted light to avoid contamination. We then outline the …
gb (code pays fourni par la source)
Yeasmin Akther, Claire Adams, Vikram Kumar Sharma, Claudia S. Barros et autres
Abstract Aims Meningioma is the most common primary intracranial tumour. Although ~80% are benign WHO grade I and show high rates of recurrence. Surgery is the main therapeutic approach, yet location can hamper complete resection and chemotherapies are ineffective. Moreover, accurate biomarkers …
gb (code pays fourni par la source)
Yeasmin Akther, Claire Adams, Vikram Kumar Sharma, Claudia S. Barros et autres
Abstract Introduction Meningioma are the most common primary intracranial tumour. According to WHO, ~80% tumours are benign grade I. Although, some grade I tumour clinically show aggressive behaviour. Radio-surgery are the main therapeutic approaches, chemotherapies are ineffective. Accurate biomarkers for clinical management …
gb (code pays fourni par la source)
Claudia S. Barros, Torsten Bossing
Repair after traumatic injury often starts with mitotic activation around the lesion edges. Early midline cells in the Drosophila embryonic CNS can enter into division following the traumatic disruption of microtubules. We demonstrate that microtubule disruption activates non-canonical TNF signaling by phosphorylation …
gb (code pays fourni par la source)
Jon Gil-Ranedo, Eleanor Gonzaga, Karolina J. Jaworek, Christian Berger et autres
Adult stem cells reactivate from quiescence to maintain tissue homeostasis and in response to injury. How the underlying regulatory signals are integrated is largely unknown. Drosophila neural stem cells (NSCs) also leave quiescence to generate adult neurons and glia, a process that …
gb, de (code pays fourni par la source)
Shamprasad Varija Raghu, Farhan Mohammad, Jia Yi Chua, Joanne Shi Woon Lam et autres
The analysis of behavior requires that the underlying neuronal circuits are identified and genetically isolated. In several major model species-most notably Drosophila-neurogeneticists identify and isolate neural circuits with a binary heterologous expression-control system: Gal4-UASG. One limitation of Gal4-UASG is that expression patterns …
sg, in, gb (code pays fourni par la source)
Shamprasad Varija Raghu, Farhan Mohammad, Jia Yi Chua, Claudia S. Barros et autres
Abstract The analysis of behavior requires that the underlying neuronal circuits are identified and genetically isolated. In several major model species—most notably Drosophila , neurogeneticists identify and isolate neural circuits with a binary heterologous expression-control system: Gal4–UASG. One limitation of Gal4–UASG is …
sg, gb (code pays fourni par la source)
Shamprasad Varija Raghu, Farhan Mohammad, Jia Yi Chua, Claudia S. Barros et autres
This is microscopy image data and related information for A zinc-finger fusion protein refines Gal4-defined neural circuits The analysis of behavior requires that the underlying neuronal circuits are identified and genetically isolated. In several major model species—most notably Drosophila, neurogeneticists identify and …
sg (code pays fourni par la source)
Shamprasad Varija Raghu, Farhan Mohammad, Jia Yi Chua, Claudia S. Barros et autres
This is microscopy image data and related information for A zinc-finger fusion protein refines Gal4-defined neural circuits The analysis of behavior requires that the underlying neuronal circuits are identified and genetically isolated. In several major model species—most notably Drosophila, neurogeneticists identify and …
sg (code pays fourni par la source)
Jin Xu, Brigham J. Hartley, Pradeep K. Kurup, André W. Phillips et autres
The brain-specific tyrosine phosphatase, STEP (STriatal-Enriched protein tyrosine Phosphatase) is an important regulator of synaptic function. STEP normally opposes synaptic strengthening by increasing N -methyl D -aspartate glutamate receptor (NMDAR) internalization through dephosphorylation of GluN2B and inactivation of the kinases extracellular signal–regulated …
us, gb (code pays fourni par la source)
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