Gabriele Spohn

Cryopreservation of mesenchymal stem/stromal cells using a DMSO-free solution is comparable to DMSO-containing cryoprotectants: results of an international multicenter PACT/BEST collaborative study

Tewodros Mamo, Cheryl A. Cox, Connor Demorest, Magali J. Fontaine et autres

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Inter- and Intra-donor variability in bone marrow–derived mesenchymal stromal cells: implications for clinical applications

Alpa A. Trivedi, Maximillian Lin, Byron Y. Miyazawa, Alison Nair et autres

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Small volume bone marrow aspirates with high progenitor cell concentrations maximize cell therapy dose manufacture and substantially reduce donor hemoglobin loss

Jeremy Epah, Gabriele Spohn, Kathrin Preiß, Markus Matthias Müller et autres

BACKGROUND: Bone marrow (BM) transplantation is a life-saving therapy for hematological diseases, and the BM harbors also highly useful (progenitor) cell types for novel cell therapies manufacture. Yet, the BM collection technique is not standardized. METHODS: Benchmarking our collection efficiency to BM …

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Mesenchymal Stem/Stromal Cells: AN INVESTIGATION OF INTER- AND INTRA-DONOR VARIABILITY IN BONE MARROW-DERIVED MESENCHYMAL STROMAL CELLS: IMPLICATIONS FOR CLINICAL APPLICATIONS

Alpa A. Trivedi, Mao Lin, Byron Y. Miyazawa, Ashwin Nair et autres

Mesenchymal Stem/Stromal Cells: POOLED HUMAN BONE MARROW-DERIVED MSCS IMPROVE WOUND HEALING IN DIABETIC RATS BY SUPPORTING WOUND VASCULARIZATION, COLLAGEN DEPOSITION AND INDUCING M2-LIKE MACROPHAGE RECRUITMENT

Hélène Willer, Corinna Thielemann, Susanne Elvers-Hornung, Gabriele Spohn et autres

Regulation of CRE-Dependent Transcriptional Activity in a Mouse Suprachiasmatic Nucleus Cell Line

Monica Langiu, Philipp Bechstein, Gabriele Spohn, Erik Maronde

We evaluated the signalling framework of immortalized cells from the hypothalamic suprachiasmatic nucleus (SCN) of the mouse. We selected a vasoactive intestinal peptide (VIP)-positive sub-clone of immortalized mouse SCN-cells stably expressing a cAMP-regulated-element (CRE)-luciferase construct named SCNCRE. We characterized these cells in …

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Pooled human bone marrow-derived mesenchymal stromal cells with defined trophic factors cargo promote dermal wound healing in diabetic rats by improved vascularization and dynamic recruitment of M2-like macrophages

Hélène Willer, Gabriele Spohn, Kimberly Morgenroth, Corinna Thielemann et autres

Human Mesenchymal Stromal Cells (hMSCs) are a promising source for cell-based therapies. Yet, transition to phase III and IV clinical trials is remarkably slow. To mitigate donor variabilities and to obtain robust and valid clinical data, we aimed first to develop a …

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Repeated administration of human bone marrow derived MSC expanded in virally inactivated human platelet lysate improves wound healing in diabetic rats

Hélène Willer, Corinna Thielemann, Susanne Elvers-Hornung, Gabriele Spohn et autres

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More Human BM-MSC With Similar Subpopulation Composition and Functional Characteristics Can Be Produced With a GMP-Compatible Fabric Filter System Compared to Density Gradient Technique

Gabriele Spohn, Anne-Sophie Witte, Anja Kretschmer, Erhard Seifried et autres

Background Mesenchymal stromal cells (MSCs), multipotent progenitors that can be isolated from a variety of different tissues, are becoming increasingly important as cell therapeutics targeting immunopathologies and tissue regeneration. Current protocols for MSC isolation from bone marrow (BM) rely on density gradient …

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Adaptive Immunity and Pathogenesis of Diabetes: Insights Provided by the α4–Integrin Deficient NOD Mouse

Salim Oulghazi, Sarah K. Wegner, Gabriele Spohn, Nina Müller et autres

BACKGROUND: The spontaneously diabetic "non-obese diabetic" (NOD) mouse is a faithful model of human type-1 diabetes (T1D). METHODS: Given the pivotal role of α4 integrin (CD49d) in other autoimmune diseases, we generated NOD mice with α4-deficient hematopoiesis (NOD.α4-/-) to study the role …

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Modulating endothelial adhesion and migration impacts stem cell therapies efficacy

Richard Schäfer, Matthias Georg Schwab, Georg Siegel, Andreas von Ameln-Mayerhofer et autres

BACKGROUND: Limited knowledge of stem cell therapies` mechanisms of action hampers their sustainable implementation into the clinic. Specifically, the interactions of transplanted stem cells with the host vasculature and its implications for their therapeutic efficacy are not elucidated. We tested whether adhesion …

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Human Mesenchymal Stromal Cells Are Resistant to SARS-CoV-2 Infection under Steady-State, Inflammatory Conditions and in the Presence of SARS-CoV-2-Infected Cells

Richard Schäfer, Gabriele Spohn, Marco Bechtel, Denisa Bojková et autres

Previous studies reported on the safety and applicability of mesenchymal stem/stromal cells (MSCs) to ameliorate pulmonary inflammation in acute respiratory distress syndrome (ARDS). Thus, multiple clinical trials assessing the potential of MSCs for COVID-19 treatment are underway. Yet, as SARS-inducing coronaviruses infect …

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