Yoshio Yamamoto

A serum-reduced culture system for canine PBMC-derived macrophages enables characterization of M0, M1, and M2 phenotypes and tumor-associated interactions

Yuki Hoshino, Riho Anzai, Saori Kobayashi, Naohiro Uchida et autres

Canine macrophages are increasingly recognized as important regulators of tumor immunity; however, in vitro culture systems that minimize serum-derived variability remain limited. In this study, we established a serum-reduced culture system for canine peripheral blood mononuclear cell (PBMC)-derived macrophages and characterized their …

jp (code pays fourni par la source)

Shifts in type 2 vomeronasal receptor expression during postnatal development in the lungfish olfactory organ

Shoko Nakamuta, Zicong Zhang, Masato Nikaido, Takuya Yokoyama et autres

Many tetrapods possess two distinct olfactory organs: the olfactory epithelium (OE) and the vomeronasal organ (VNO). Fish have only the OE, but lungfish-the closest living relative of tetrapods among fish- possess a lamellar OE and a primitive VNO called a recess epithelium …

jp (code pays fourni par la source)

Postnatal developmental changes in the laryngeal chemosensory cell clusters of rats

Sayed Sharif Abdali, Kanna Miyazaki, Takuya Yokoyama, Nobuaki Nakamuta et autres

Chemosensory cell clusters are taste bud-like sensory structures located in the pharyngo-laryngeal mucosa. These clusters are densely distributed at the entrance of lower airways to detect chemical stimuli and trigger respiratory reflexes; however, their postnatal development remains poorly understood. In this study, …

jp (code pays fourni par la source)

Re‐Evaluating the Population History of Malagasy Native Cattle Reveals Shared African Taurine‐Related Ancestry but Distinct Indicine Admixture Histories Relative to East African Cattle

Jiaqi Wu, Hideki Endo, Felix Rakotondraparany, Fanomezana Mihaja Ratsoavina et autres

ABSTRACT East African cattle, including Malagasy native cattle (ZMA), are generally thought to have been shaped by admixture between African taurine and Asian indicine lineages, but the detailed position and formation history of ZMA within this broader diversity remain unclear. We analyzed …

jp, Madagascar (code pays fourni par la source)

Immunohistochemical distribution of extracellular signal-regulated kinase 1 and 2 (ERK1/2) in the rat carotid body

Hiroki Saito, Takuya Yokoyama, Nobuaki Nakamuta, Yoshio Yamamoto

The sensitivity of the carotid body to hypoxic stimuli is enhanced by chronic intermittent hypoxia. Since extracellular signal-regulated kinase 1/2 (ERK1/2) may be involved in hypoxic responses and in sensory nerve facilitation induced by intermittent hypoxic stimuli, we examined the distribution of …

jp (code pays fourni par la source)

Increased Fos immunoreactivity in astrocytes in the raphe pallidus under hypoxia, not hypercapnia

Kouki Kato, Risa Serizawa, Takuya Yokoyama, Nobuaki Nakamuta et autres

The raphe pallidus (RPa), a part of the caudal medullary raphe nucleus, has been suggested to participate in respiratory regulation. Therefore, hypoxia and hypercapnia are expected to affect the expression of Fos, a marker of cellular activation, in the RPa; however, there …

jp (code pays fourni par la source)

Interrelationship Between Intraepithelial Nerve Endings and Epithelial Cells in the Rat Epiglottis Revealed by Array Tomography With Scanning Electron Microscopy

Yoshio Yamamoto, Kuniaki Sasaki, Misaki Komuro, Takuya Yokoyama et autres

Nerve endings in the laryngeal mucosa interact with epithelial cells to generate sensory discharges against various external stimuli. In the present study, we performed array tomography with scanning electron microscopy to examine the morphological interrelationship between intraepithelial nerve endings and epithelial cells …

jp (code pays fourni par la source)

WS05.02CFTR mRNA delivery with a revolutionary non-LNP nanoemulsion formulation to differentiated primary human airway epithelium and airway organoid

Kenjiro Ito, Shyreen Hassibi, Yoshio Yamamoto, Jag Shur et autres

gb (code pays fourni par la source)

Fos expression in A1/C1 neurons of rats exposed to hypoxia, hypercapnia, or hypercapnic hypoxia

Kouki Kato, Risa Serizawa, Takuya Yokoyama, Nobuaki Nakamuta et autres

The distribution of Fos expression in catecholaminergic neurons with immunoreactivity for dopamine β-hydroxylase (DBH) of the ventrolateral medulla was compared between rats exposed to hypoxia (10 % O 2 ), hypercapnia (8 % CO 2 ), and hypercapnic hypoxia (8 % CO …

jp (code pays fourni par la source)

Immunohistochemical distribution of cannabinoid receptor type 1 (CB1) and type 2 (CB2) in the rat carotid body

Hiroki Saito, Takuya Yokoyama, Nobuaki Nakamuta, Yoshio Yamamoto

The carotid body is a hypoxia-sensitive chemoreceptor that induces sensory long-term facilitation after exposure to chronic intermittent hypoxia . However, the mechanisms underlying synaptic plasticity in the carotid body remain unknown. In the present study, we examined the immunohistochemical distribution of cannabinoid …

jp (code pays fourni par la source)

Type 2 vomeronasal receptor expression in the olfactory organ of African lungfish, Protopterus annectens

Shoko Nakamuta, Zicong Zhang, Masato Nikaido, Takuya Yokoyama et autres

jp (code pays fourni par la source)

Distribution and morphology of serotonin transporter‐immunoreactive type I cells in the rat carotid body

Takuya Yokoyama, Sayed Sharif Abdali, Nobuaki Nakamuta, Yoshio Yamamoto

The present study reexamined the immunolocalization of membranous serotonin transporter (SERT) in the rat carotid body, and demonstrated SERT-immunoreactive cells of unreported morphology. SERT was immunohistochemically localized in a very small population of cell clusters or single type I cells (2.8%) immunoreactive …

jp (code pays fourni par la source)