Naohiro Uchida

A serum-reduced culture system for canine PBMC-derived macrophages enables characterization of M0, M1, and M2 phenotypes and tumor-associated interactions

Yuki Hoshino, Riho Anzai, Saori Kobayashi, Naohiro Uchida et autres

Canine macrophages are increasingly recognized as important regulators of tumor immunity; however, in vitro culture systems that minimize serum-derived variability remain limited. In this study, we established a serum-reduced culture system for canine peripheral blood mononuclear cell (PBMC)-derived macrophages and characterized their …

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Independent clonal evolution in primary pigmented nodular adrenocortical disease: a single-patient multi-omics study

Tazuru Fukumoto, Hironobu Umakoshi, Norifusa Iwahashi, Tatsuki Ogasawara et autres

OBJECTIVE: Primary pigmented nodular adrenocortical disease (PPNAD), caused by germline mutations of PRKAR1A encoding the regulatory subunit 1A of protein kinase A (PKA), exhibits marked lesion heterogeneity, ranging from non-tumorous adrenocortical tissue to multiple micronodular and occasionally adenomatous cortisol-producing lesions within the …

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A case of obstructive jaundice caused by a pancreatic pseudocyst successfully treated with pancreatic duct stenting

Takuya Ishida, Masao Toki, Naohiro Uchida, Yuta Sato et autres

A man in his seventies presented to our hospital with obstructive jaundice. Contrast-enhanced abdominal computed tomography revealed a unilocular cystic lesion, measuring 53 × 47 mm in the pancreatic head, accompanied by distal bile duct stricture and upstream biliary dilatation. Endoscopic retrograde …

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Antibacterial spectrum of diaphorin, a polyketide produced by a bacterial symbiont of the Asian citrus psyllid

Takashi Izu, Naohiro Uchida, Rena Takasu, Atsushi Nakabachi

Diaphorin is a polyketide synthesized by "Candidatus Profftella armatura" (Betaproteobacteria: Burkholderiales), an obligate symbiont of a devastating agricultural pest, the Asian citrus psyllid Diaphorina citri (Hemiptera: Psyllidae). Previous studies showed that physiological concentrations of diaphorin, which is present in D. citri at …

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Antibacterial Spectrum of Diaphorin, a Polyketide Produced by a Bacterial Symbiont of the Asian Citrus Psyllid

Atsushi Nakabachi, Takashi Izu, Naohiro Uchida, Rena Takasu

Single-cell and spatial transcriptomics analysis of human adrenal aging

Norifusa Iwahashi, Hironobu Umakoshi, Masamichi Fujita, Tazuru Fukumoto et autres

OBJECTIVE: The human adrenal cortex comprises three functionally and structurally distinct layers that produce layer-specific steroid hormones. With aging, the human adrenal cortex undergoes functional and structural alteration or "adrenal aging", leading to the unbalanced production of steroid hormones. Given the marked …

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Steroids-producing nodules: a two-layered adrenocortical nodular structure as a precursor lesion of cortisol-producing adenoma

Tazuru Fukumoto, Hironobu Umakoshi, Norifusa Iwahashi, Tatsuki Ogasawara et autres

BACKGROUND: The human adrenal cortex consists of three functionally and structurally distinct layers; zona glomerulosa, zona fasciculata (zF), and zona reticularis (zR), and produces adrenal steroid hormones in a layer-specific manner; aldosterone, cortisol, and adrenal androgens, respectively. Cortisol-producing adenomas (CPAs) occur mostly …

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Effects of chemotherapy treatment with doxorubicin on right ventricular function in dogs

Tomoya Morita, Naohiro Uchida, Mayu KIMURA

Left ventricular dysfunction in dogs after the administration of doxorubicin (DOX) has been extensively examined. However, the effects of DOX on right ventricular (RV) function remain unknown. Therefore, the present study investigated whether the chemotherapy treatment with DOX decreases RV function. Twelve …

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Dectin-2 Deficiency Promotes Proinflammatory Cytokine Release From Macrophages and Impairs Insulin Secretion

Masamichi Fujita, Takashi Miyazawa, Keiichiro Uchida, Naohiro Uchida et autres

Pancreatic islet inflammation plays a crucial role in the etiology of type 2 diabetes (T2D). Macrophages residing in pancreatic islets have emerged as key players in islet inflammation. Macrophages express a plethora of innate immune receptors that bind to environmental and metabolic …

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252-LB: Dectin-2 Deficiency Promotes Proinflammatory Cytokine Release from Macrophages and Impairs Glucose-Stimulated Insulin Secretion

Masamichi Fujita, Takashi Miyazawa, Keiichiro Uchida, Naohiro Uchida et autres

Pancreatic islet inflammation plays an important role in the pathogenesis of diabetes mellitus. Islet macrophages, which have emerged as a key player in islet inflammation, express Dectin-2 (D2), one of the C-type lectin receptors recognizing α-mannans, while endocrine cells such as α-cells …

The role of heat shock protein 90 in the proliferation of Babesia gibsoni in vitro

Hiroka Tsutsumi, Moeko Abe, Naohiro Uchida, Mitsuyoshi Takiguchi et autres

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Vitamin C transporter SVCT1 serves a physiological role as a urate importer: functional analyses and in vivo investigations

Yu Toyoda, Hiroshi Miyata, Naohiro Uchida, Keito Morimoto et autres

Uric acid, the end product of purine metabolism in humans, is crucial because of its anti-oxidant activity and a causal relationship with hyperuricemia and gout. Several physiologically important urate transporters regulate this water-soluble metabolite in the human body; however, the existence of …

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