Tatjana Schütze

RNAi-mediated knockdown of the Rhau helicase preferentially depletes proteins with a Guanine-quadruplex motif in the 5'-UTR of their mRNA

Karen Vester, Murat Eravci, Tatsuo Serikawa, Tatjana Schütze et autres

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Identification and characterization of RNA guanine-quadruplex binding proteins

Annekathrin von Hacht, Oliver Seifert, Marcus Menger, Tatjana Schütze et autres

Guanine quadruplex (G-quadruplex) motifs in the 5' untranslated region (5'-UTR) of mRNAs were recently shown to influence the efficiency of translation. In the present study, we investigate the interaction between cellular proteins and the G-quadruplexes located in two mRNAs (MMP16 and ARPC2). …

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SELEX-Technologieentwicklung im Hochdurchsatz

Tatjana Schütze

Der SELEX-Prozess zur Selektion funktioneller Nukleinsäuren (hier: Aptamere) ist, obgleich mittlerweile vielfach modifiziert, noch immer ein zeit- und damit kostenintensives Verfahren. Eine parallele Selektion von DNA-Aptameren in 10 Runden gegen Streptavidin wurde halbautomatisiert, und zunächst nach herkömmlichen Protokollen (Klonierung, Sanger- Sequenzierung), durchgeführt …

Probing the SELEX Process with Next-Generation Sequencing

Tatjana Schütze, Barbara Wilhelm, Nicole Greiner, Hannsjörg Braun et autres

BACKGROUND: SELEX is an iterative process in which highly diverse synthetic nucleic acid libraries are selected over many rounds to finally identify aptamers with desired properties. However, little is understood as how binders are enriched during the selection course. Next-generation sequencing offers …

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Analysis of Different DNA Vaccines for Protection of Experimental Influenza A Virus Infection

Nadine Wiesener, Tatjana Schütze, Sara Lapp, Melissa Lehmann et autres

Influenza viruses cause acute respiratory infections in humans that result in significant excessive morbidity and mortality rates every year. Current vaccines are limited in several aspects, including laborious manufacturing technology, non-sufficient efficacy, and time-consuming adjustments to new emerging virus variants. An alternative …

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Diversity visualization by endonuclease: A rapid assay to monitor diverse nucleotide libraries

Theam Soon Lim, Tatjana Schütze, Hans R. Lehrach, Jörn Glökler et autres

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A streamlined protocol for emulsion polymerase chain reaction and subsequent purification

Tatjana Schütze, Florian Rubelt, Julia Repkow, Nicole Greiner et autres

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Automation in the High-throughput Selection of Random Combinatorial Libraries—Different Approaches for Select Applications

Jörn Glökler, Tatjana Schütze, Zoltán Konthur

Automation in combination with high throughput screening methods has revolutionised molecular biology in the last two decades. Today, many combinatorial libraries as well as several systems for automation are available. Depending on scope, budget and time, a different combination of library and …

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A calibrated diversity assay for nucleic acid libraries using DiStRO—a Diversity Standard of Random Oligonucleotides

Tatjana Schütze, Peter F. Arndt, Marcus Menger, Aniela Wochner et autres

We have determined diversities exceeding 10(12) different sequences in an annealing and melting assay using synthetic randomized oligonucleotides as a standard. For such high diversities, the annealing kinetics differ from those observed for low diversities, favouring the remelting curve after annealing as …

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