Giovanni Pasquini

Author Correction: Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screening

Anand Ramani, Giovanni Pasquini, Niklas J. Gerkau, Vaibhav Jadhav et autres

The original version of this Article contained an error in Fig. 6Ai in which the image for the IMR90 Day 30 condition inadvertently used the image of the IMR90 Day 15 condition presented in Fig. 1G. Figure 6Ai has been updated with …

de, fr, it, us, il, no (code pays fourni par la source)

Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screening

Anand Ramani, Giovanni Pasquini, Niklas J. Gerkau, Vaibhav Jadhav et autres

Brain organoids offer unprecedented insights into brain development and disease modeling and hold promise for drug screening. Significant hindrances, however, are morphological and cellular heterogeneity, inter-organoid size differences, cellular stress, and poor reproducibility. Here, we describe a method that reproducibly generates thousands …

de, fr, it, us, il, no (code pays fourni par la source)

Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion, and drug screening

Anand Ramani, Giovanni Pasquini, Niklas J. Gerkau, Omkar Suhas Vinchure et autres

SUMMARY Brain organoids offer unprecedented insights into brain development and disease modeling and hold promise for drug screening. Significant hindrances, however, are morphological and cellular heterogeneity, inter-organoid size differences, cellular stress, and poor reproducibility. Here, we describe a method that reproducibly generates …

de, bg, fr, it, us, no (code pays fourni par la source)

Cellular senescence promotes progenitor cell expansion during axolotl limb regeneration

Qinghao Yu, Hannah Walters, Giovanni Pasquini, Sumeet Pal Singh et autres

Axolotl limb regeneration is accompanied by the transient induction of cellular senescence within the blastema, the structure that nucleates regeneration. The precise role of this blastemal senescent cell (bSC) population, however, remains unknown. Here, through a combination of gain- and loss-of-function assays, …

de, be, es (code pays fourni par la source)

Dissecting DSB-repair pathways and cell-identity by transcriptomics

Giovanni Pasquini

The analysis of the transcriptome enables the mapping of pathway activity and cell type identity across various contexts, throughout the use of diverse tools. Notably, RNA sequencing (RNA-seq) facilitates an unbiased quantification of RNA species abundance. This study focuses on a computational …

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia

Elke Gabriel, Walid Albanna, Giovanni Pasquini, Anand Ramani et autres

de, it, us, fr (code pays fourni par la source)

Reliability of High-Quantity Human Brain Organoids for Modeling Microcephaly, Glioma Invasion, and Drug Screening

Anand Ramani, Giovanni Pasquini, Niklas J. Gerkau, Nazlican Altinisk et autres

de, fr, it, us, no (code pays fourni par la source)

Cellular senescence promotes progenitor cell expansion during axolotl limb regeneration

Qinghao Yu, Hannah Walters, Giovanni Pasquini, Sumeet Pal Singh et autres

SUMMARY Axolotl limb regeneration is accompanied by the transient induction of cellular senescence within the blastema, the structure which nucleates regeneration. The precise role of this blastemal senescent cell (bSC) population, however, remains unknown. Here, through a combination of gain- and loss-of-function …

de, be, es (code pays fourni par la source)

Neuronal Cell-type Engineering by Transcriptional Activation

Songlei Liu, Johannes Striebel, Giovanni Pasquini, Alex H. M. Ng et autres

Gene activation with the CRISPR-Cas system has great implications in studying gene function, controlling cellular behavior, and modulating disease progression. In this review, we survey recent studies on targeted gene activation and multiplexed screening for inducing neuronal differentiation using CRISPR-Cas transcriptional activation …

us, de (code pays fourni par la source)

Human brain organoids assemble functionally integrated bilateral optic vesicles

Elke Gabriel, Walid Albanna, Giovanni Pasquini, Anand Ramani et autres

de, us, it, fr (code pays fourni par la source)

Human brain organoids assemble functionally integrated bilateral optic vesicles

Elke Gabriel, Walid Albanna, Giovanni Pasquini, Anand Ramani et autres

During embryogenesis, optic vesicles develop from the diencephalon via a complex process of organogenesis. Using iPSC-derived human brain organoids, we attempted to simplify the complexities and demonstrate the formation of forebrain-associated bilateral optic vesicles, cellular diversity, and functionality. Around day thirty, brain …

de, us, it, fr (code pays fourni par la source)

MiRNA Regulatory Functions in Photoreceptors

Julia Sophie Pawlick, Marta Žužić, Giovanni Pasquini, Anka Swiersy et autres

MicroRNAs (miRNAs) are important regulators of gene expression. These small, non-coding RNAs post-transcriptionally silence messenger RNAs (mRNAs) in a sequence-specific manner. In this way, miRNAs control important regulatory functions, also in the retina. If dysregulated, these molecules are involved in several retinal …

de (code pays fourni par la source)