Zdeněk Knejzlı́k

Source data for: "Inhibition of PurF activity induces conditional thiamine auxotrophy in mycobacteria"

Zdeněk Knejzlı́k

OVERVIEW This repository contains the raw source data for the growth curves presented in the associated publication. The datasets track the growth of various bacterial strains under specific experimental conditions by measuring optical density at 600 nm (OD600) over time. ASSOCIATED PUBLICATION …

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Source data for: "Inhibition of PurF activity induces conditional thiamine auxotrophy in mycobacteria"

Zdeněk Knejzlı́k

OVERVIEW This repository contains the raw source data for the growth curves presented in the associated publication. The datasets track the growth of various bacterial strains under specific experimental conditions by measuring optical density at 600 nm (OD600) over time. ASSOCIATED PUBLICATION …

cz (code pays fourni par la source)

Structural basis for allosteric regulation of mycobacterial guanosine 5´-monophosphate reductase by ATP and GTP

Michal Doležal, Zdeněk Knejzlı́k, Tomáš Kouba, Anatolij Filimoněnko et autres

Guanosine 5′-monophosphate reductase (GMPR) is a crucial enzyme in the purine salvage pathway that catalyses the NADPH-dependent conversion of GMP to IMP, thereby contributing to purine nucleotide homeostasis. Mycobacterium smegmatis GMPR (MsmGMPR) contains a regulatory cystathionine β-synthase (CBS) domain, which mediates allosteric …

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Accelerated characterization of active natural extracts with single-step microfractionation coupled to mass spectrometry and bioassays

Joshua David Smith, Erik Bouchal, Zdeněk Knejzlı́k, Martin Dračínský et autres

Microfractionation is a prominent alternative for the discovery and characterization of bioactive natural products in complex extracts (e.g., plant, fungal, or microbial); however, the method faces limitations as it requires arduous scale-up for complete characterization of molecules. Furthermore, the integration of microfractionation …

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Thiosulfonate‐Derived BODIPY “Stick and Glue” Strategy for Fluorescent Thiol Labeling

David J. Dunlop, B. Buse Tütüncü, Zdeněk Knejzlı́k, Tomáš Slanina

Fluorescent thiol labeling is a powerful technique for investigating peptide and protein functions, interactions, and cellular localization, offering minimal structural disruption due to the low natural abundance of cysteine residues. The high nucleophilicity of cysteine thiol groups further facilitates efficient and selective …

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Thiosulfonate‐Derived BODIPY “Stick and Glue” Strategy for Fluorescent Thiol Labeling

David J. Dunlop, B. Buse Tütüncü, Zdeněk Knejzlı́k, Tomáš Slanina

Abstract Fluorescent thiol labeling is a powerful technique for investigating peptide and protein functions, interactions, and cellular localization, offering minimal structural disruption due to the low natural abundance of cysteine residues. The high nucleophilicity of cysteine thiol groups further facilitates efficient and …

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Structural basis for allosteric regulation of mycobacterial guanosine 5´-monophosphate reductase by ATP and GTP

Iva Pichová, Michal Doležal, Zdeněk Knejzlı́k, Tomáš Kouba et autres

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Conformational landscape of the mycobacterial inosine 5′-monophosphate dehydrogenase octamerization interface

Ondřej Bulvas, Zdeněk Knejzlı́k, Anatolij Filimoněnko, Tomáš Kouba et autres

• Cryo-EM reveals the E-XMP* reaction intermediate of full-length Msm IMPDH. • Binding of IMP alone induces K + binding site formation and C-terminal folding. • A novel octamerization interface is observed in the compressed Msm IMPDH structure. • C-terminal regions self-interact, …

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Conformational Landscape of the Mycobacterial Inosine-5′-Monophosphate Dehydrogenase Octamerization Interface

Ondřej Bulvas, Zdeněk Knejzlı́k, Anatolij Filimoněnko, Tomáš Kouba et autres

HelD is a Global Transcription Factor Enhancing Gene Expression in Rapidly Growing Mycobacteria

Viola Vaňková Hausnerová, Dilip Kumar, Mahmoud Shoman, Marek Schwarz et autres

ABSTRACT HelD protein, also named HelR (encoded by MSMEG_2174 in Mycobacterium smegmatis ), interacts with mycobacterial RNA polymerase (RNAP) and affects rifampicin resistance in Mycobacterium abscessus . Here, we provide data on rifampicin resistance and helD presence in the genomes of other …

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Deciphering the allosteric regulation of mycobacterial inosine-5′-monophosphate dehydrogenase

Ondřej Bulvas, Zdeněk Knejzlı́k, Jakub Sýs, Anatolij Filimoněnko et autres

Allosteric regulation of inosine 5'-monophosphate dehydrogenase (IMPDH), an essential enzyme of purine metabolism, contributes to the homeostasis of adenine and guanine nucleotides. However, the precise molecular mechanism of IMPDH regulation in bacteria remains unclear. Using biochemical and cryo-EM approaches, we reveal the …

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Design and evaluation of composite films for in situ synthesis and antibacterial activity of allicin vapour

Lucie Mašková, Lenka Závišová, Ondřej Kašpar, Zdeněk Knejzlı́k et autres

Abstract Although allicin has potent antibiotic properties, its low stability, which is responsible for its persistent biological activity, has posed a significant challenge to its practical application in modern medicine. To harness the healing benefits of this phytochemical, known by humans for …

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