Michal Doležal

Highly efficient base editing at PCSK9 and normal human embryo development

Stepan Jerabek, Chanju Jung, Michelle Kappy, Qiaojin Zhao et autres

cz, us, kr (code pays fourni par la source)

Structural basis for allosteric regulation of mycobacterial guanosine 5´-monophosphate reductase by ATP and GTP

Michal Doležal, Zdeněk Knejzlı́k, Tomáš Kouba, Anatolij Filimoněnko et autres

Guanosine 5′-monophosphate reductase (GMPR) is a crucial enzyme in the purine salvage pathway that catalyses the NADPH-dependent conversion of GMP to IMP, thereby contributing to purine nucleotide homeostasis. Mycobacterium smegmatis GMPR (MsmGMPR) contains a regulatory cystathionine β-synthase (CBS) domain, which mediates allosteric …

cz (code pays fourni par la source)

Hepatitis B virus is a stealth virus that minimizes proteomic and secretomic changes in primary human hepatocytes

Karolína Štaflová, Kamila Clarová, Michal Doležal, Martin Hubálek et autres

Hepatitis B virus (HBV) is a hepatotropic DNA virus that infects over 250 million people worldwide and causes serious liver diseases. HBV infection can modulate host cellular processes, potentially inducing proteomic changes in hepatocytes. In this study, we investigated how acute HBV …

cz (code pays fourni par la source)

Structural basis for allosteric regulation of mycobacterial guanosine 5´-monophosphate reductase by ATP and GTP

Iva Pichová, Michal Doležal, Zdeněk Knejzlı́k, Tomáš Kouba et autres

cz (code pays fourni par la source)

The mycobacterial guaB1 gene encodes a guanosine 5′‐monophosphate reductase with a cystathionine‐β‐synthase domain

Zdeněk Knejzlı́k, Michal Doležal, Klára Herkommerová, Kamila Clarová et autres

Mycobacteria express enzymes from both the de novo and purine-salvage pathways. However, the regulation of these processes and the roles of individual metabolic enzymes have not been sufficiently detailed. Both Mycobacterium tuberculosis (Mtb) and Mycobacterium smegmatis (Msm) possess three guaB genes, but …

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Author response for "The mycobacterial guaB1 gene encodes a guanosine 5´‐monophosphate reductase with a cystathionine‐β‐synthase domain"

Zdeněk Knejzlı́k, Michal Doležal, Klára Herkommerová, Kamila Clarová et autres

The mycobacterial guaB1 gene encodes a guanosine 5’-monophosphate reductase with a cystathione-β-synthase domain

Zdeněk Knejzlı́k, Michal Doležal, Klára Herkommerová, Kamila Clarová et autres

Abstract Purine metabolism plays a pivotal role in bacterial life cycle, however, regulation of the de novo and purine salvage pathways have not been extensively detailed in mycobacteria. By gene knockout, biochemical and structural analyses, we identified Mycobacterium smegmatis (Msm) and Mycobacterium …

cz (code pays fourni par la source)

Víceúčelová optimalizace betonové konstrukce v rané fázi návrhu

Michal Doležal

Diplomová práce se zabývá využitím parametrického modelování a genetických algoritmů pro víceúčelovou optimalizaci v rané fázi návrhu betonové konstrukce. Tyto moderní metody umožňují zlepšení spolupráce více profesí v jednom modelu vytvořeném v programovém prostředí. Dále zprostředkují společný přístup k tvorbě budovy jako …

Hepatitis B Virus X Protein Function Requires Zinc Binding

Dhivya Ramakrishnan, Weimei Xing, Rudolf K. Beran, Saketh Chemuru et autres

The structural maintenance of chromosomes 5/6 complex (Smc5/6) is a host restriction factor that suppresses HBV transcription. HBV counters this restriction by expressing HBV X protein (HBx), which redirects a host ubiquitin ligase to target Smc5/6 for degradation. Despite this recent advance …

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Structure and architecture of immature and mature murine leukemia virus capsids

Kun Qu, Bärbel Glass, Michal Doležal, F.K.M. Schur et autres

Retroviruses assemble and bud from infected cells in an immature form and require proteolytic maturation for infectivity. The CA (capsid) domains of the Gag polyproteins assemble a protein lattice as a truncated sphere in the immature virion. Proteolytic cleavage of Gag induces …

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Immature M-PMV capsid hexamer structure in intact virus particles

Kun Qu, Bärbel Glass, Michal Doležal, F.K.M. Schur et autres

Structure of murine leukemia virus capsid C-terminal domain

Kun Qu, Michal Doležal, F.K.M. Schur, Brice Murciano et autres

Retroviruses assemble and bud from infected cells in an immature form and require proteolytic maturation for infectivity. The CA (capsid) domains of the Gag polyproteins assemble a protein lattice as a truncated sphere in the immature virion. Proteolytic cleavage of Gag induces …

de, cz, at, us (code pays fourni par la source)