Chris Van den Haute

Protein aggregation inhibitors induce divergent transcriptional responses in a cellular model of ɑ-synuclein seeded aggregation

Paulien Van Minsel, Chris Van den Haute, Eline Vonck, Shirine Hentati et autres

Abstract Parkinson’s disease (PD), dementia with Lewy Bodies (DLB) and multiple system atrophy (MSA) are progressive neurodegenerative disorders marked by the pathological aggregation of alpha-synuclein (ɑSyn). Despite significant research efforts, effective therapeutic interventions remain elusive due to limited understanding of the cellular …

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ATP13A4 gates extracellular polyamine levels to control excitatory synaptogenesis

Sarah van Veen, Emily Meeus, Dolores Irala, Kristina Sakers et autres

Polyamines, such as spermidine, are essential regulators of brain development, yet how cells control their uptake and extracellular levels remains unclear. Here we show that ATP13A4, a transport protein enriched in glia and prominently expressed in astrocytes, governs brain polyamine balance. Using …

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Analysis code for "A druggable ATP13A3–antizyme switch controls adaptive polyamine uptake in cancer "

Alain René Schoofs, Jialin Chen, Rania Abou El Asrar, Eline Ryckaert et autres

Analysis code accompanying the study "ATP13A3–antizyme control defines a targetable mechanism for polyamine depletion in cancer". R scripts analyse GSE46517 (Affymetrix U133A) and TCGA-SKCM (RNA-seq) expression in primary vs metastatic melanoma; Jupyter notebooks generate the CSV files and plots for figures 6A, …

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Analysis code for "A druggable ATP13A3–antizyme switch controls adaptive polyamine uptake in cancer "

Alain René Schoofs, Jialin Chen, Rania Abou El Asrar, Eline Ryckaert et autres

Analysis code accompanying the study "ATP13A3–antizyme control defines a targetable mechanism for polyamine depletion in cancer". R scripts analyse GSE46517 (Affymetrix U133A) and TCGA-SKCM (RNA-seq) expression in primary vs metastatic melanoma; Jupyter notebooks generate the CSV files and plots for figures 6A, …

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A druggable ATP13A3–antizyme switch controls adaptive polyamine uptake in cancer

Alain René Schoofs, Jialin Chen, Rania Abou El Asrar, Eline Reyckaert et autres

Abstract Cellular polyamine depletion is a promising anticancer strategy, but compensatory polyamine uptake limits efficacy when synthesis is blocked by DFMO (difluoromethylornithine), a clinically approved inhibitor of ornithine decarboxylase. The transporter and feedback mechanism driving this adaptive response have remained unclear. Despite …

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Flow cytometry data from the manuscript "Fluorescence-Gated Flow Cytometry Approach for Measuring Lipid Flippase Activity in Mamma-lian Cells"

Klara Scholtissek, Filip Pamula, Chris Van den Haute, Joseph A. Lyons

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Absence of Ledgf in mouse brain affects the Kmt2a/b and polycomb balance, synaptic transmission and motor function

Laura Debusschere, Eduard Bentea, Cecilia Iglesias-Herrero, Nicolas Peredo et autres

Abstract Lens epithelium-derived growth factor (LEDGF), encoded by the Psip1 gene, exists in two splice variants, LEDGF/p75 and LEDGF/p52. Although little is known about its role in the brain, LEDGF has been proposed to play a role in neurogenesis. Since known LEDGF …

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Inactive ryanodine receptors sustain lysosomal availability for autophagy by promoting ER-lysosomal contact site formation

Tim Vervliet, Jens Loncke, Marko Sever, Karan Ahuja et autres

Lysosomal and endoplasmic reticulum (ER) Ca2+ release mutually influence each other’s functions. Recent work revealed that ER-located ryanodine receptor(s) (RyR(s)) Ca2+ release channels suppress autophagosome turnover by the lysosomes. In familial Alzheimer’s disease, inhibiting RyR hyperactivity restored autophagic flux by normalizing lysosomal …

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A druggable ATP13A3–antizyme switch controls adaptive polyamine uptake in cancer

Alain René Schoofs, Jialin Chen, Rania Abou El Asrar, Eline Reyckaert et autres

Cellular polyamine depletion is a promising anticancer strategy, but compensatory polyamine uptake limits efficacy when synthesis is blocked by DFMO (difluoromethylornithine), a clinically approved inhibitor of ornithine decarboxylase. The transporter and feedback mechanism driving this adaptive response have remained unclear. Despite their …

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A druggable ATP13A3–antizyme switch controls adaptive polyamine uptake in cancer

Alain René Schoofs, Jialin Chen, Rania Abou El Asrar, Eline Reyckaert et autres

Cellular polyamine depletion is a promising anticancer strategy, but compensatory polyamine uptake limits efficacy when synthesis is blocked by DFMO (difluoromethylornithine), a clinically approved inhibitor of ornithine decarboxylase. The transporter and feedback mechanism driving this adaptive response have remained unclear. Despite their …

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Impact of rAAV-shRNA treatment targeting mechanosensitive Ilk1 and Fermt2 in a mouse model of sepsis-induced muscle weakness

Alexander Pacolet, Inge Derese, Lies Pauwels, Sarah Vander Perre et autres

INTRODUCTION: Intensive-care-unit-acquired-muscle-weakness is a debilitating complication of sepsis, characterized by loss of muscle mass and functionality. Immobilization is an important trigger, but the role of disturbed mechanical signaling is incompletely understood. In health, the integrin-receptor-complex with key components Kindlin2 (KIND2/Fermt2) and integrin-linked-kinase …

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Development of convolutional neural networks for automated brain-wide histopathological analysis in mouse models of synucleinopathies

A Barber-Janer, Eva Van Acker, Eline Vonck, Dieter Plessers et autres

Preclinical animal models are indispensable for uncovering disease mechanisms and developing novel therapeutic interventions in synucleinopathies. Key readouts including neuronal cell death, neuroinflammation and alpha-synuclein protein aggregation, are routinely assessed by histological methods. However, traditional characterization of histological samples is labor-intensive and …

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