Eduard Bentea

Absence of Ledgf in mouse brain affects the Kmt2a/b and polycomb balance, synaptic transmission and motor function

Laura Debusschere, Eduard Bentea, Cecilia Iglesias-Herrero, Nicolas Peredo et autres

Abstract Lens epithelium-derived growth factor (LEDGF), encoded by the Psip1 gene, exists in two splice variants, LEDGF/p75 and LEDGF/p52. Although little is known about its role in the brain, LEDGF has been proposed to play a role in neurogenesis. Since known LEDGF …

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Intact corticostriatal function in aged system xc- - deficient mice

Laura De Pauw, Agnès Villers, Cindy Moore, Olaya Lara et autres

System x c - (with xCT as specific subunit) is an astrocytic cystine/glutamate antiporter that constitutes the major source of extracellular glutamate in the mouse striatum. We previously reported that young-adult mice lacking xCT (xCT -/- mice) display decreased intracellular glutamate levels …

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The G2019S LRRK2 mutation exacerbates α-synuclein and tau neuropathology through divergent pathways in Parkinson’s disease models

George Tsafaras, Diego Cabezudo, Lot Wetzels, Iraklis Tsakogias et autres

Aggregated α-synuclein (αSyn) is a pathological hallmark of Parkinson's disease (PD), yet other protein aggregates, including tau, are commonly observed in PD brains. This suggests that PD is not solely a synucleinopathy but may involve multiple, coexisting proteinopathies. Mutations in LRRK2, particularly …

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Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and parkinsonian motor deficits

María Sanchiz-Calvo, Elena Coccia, Christopher Cawthorne, Gustavo Morrone Parfitt et autres

Abstract ATP10B, a transmembrane lipid flippase located in late endosomes and lysosomes, facilitates the export of glucosylceramide and phosphatidylcholine by coupling this process to ATP hydrolysis. Recently, loss-of-function mutations in the ATP10B gene have been identified in Parkinson’s disease patients, pointing to …

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Immunofluorescence staining, vibratome sections v1

Eduard Bentea, María Sanchiz Calvo, Veerle Baekelandt

Protocol for performing immunofluorescence staining on free-floating vibratome cut brain sections from rats or mice.

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Catalepsy test in rats v1

Eduard Bentea, María Sanchiz Calvo, Veerle Baekelandt

Protocol for performing the catalepsy bar test in rats. This test evaluates muscular rigidity, and can be used in rat models with motor deficits, such as models of Parkison's disease.

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Western blot for tissue extract v1

María Sanchiz Calvo, Eduard Bentea, Veerle Baekelandt

Protocol for the detection of proteins by Western blot from tissue extract

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Cylinder test in rats v1

Eduard Bentea, María Sanchiz Calvo, Veerle Baekelandt

Protocol for performing the cylinder test in rats. The cylinder test evaluates asymmetry in forelimb use, and can be used in animal models with unilateral lesions of the nigrostriatal dopaminergic pathway.

Immunofluorescence staining ASE, vibratome sections v1

Eduard Bentea, María Sanchiz Calvo, Veerle Baekelandt

Protocol for performing immunofluorescence staining using antibody signal enhancement (ASE) on vibratome cut brain sections from rats or mice.

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Elevated body swing test in rats v1

Eduard Bentea, María Sanchiz Calvo, Veerle Baekelandt

Protocol for performing the elevated body swing test in rats. The elevated body swing evaluates motor asymmetry in body swings when the rat is briefly hanged by the tail, and can be used in animal models with unilateral lesions of the nigrostriatal …

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Open field test in rats v1

Eduard Bentea, María Sanchiz Calvo, Veerle Baekelandt

Protocol for performing the open field test in rats. The open field test measures spontaneous locomotion during 5-min free exploration of an open field arena (1m x 1m), and can be used to measure hypolocomotion in animal models of Parkinson's disease. Rats …

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Rotarod test in rats v1

Eduard Bentea, María Sanchiz Calvo, Veerle Baekelandt

Protocol for performing the rotarod test in rats. This test evaluated motor coordination and balance, and can be used in rat models with motor deficits, such as models of Parkison's disease. Rats with impaired motor function show a decreased latency to fall …

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