Miyuki Kawase

Continued co-circulation of multiple human parainfluenza virus type 3 lineages in Fukushima and Miyagi, Japan in 2025

Kazuya Shirato, Miyuki Kawase, Satoko Sugimoto, Masatoshi Kakizaki et autres

In Japan, nationwide surveillance of acute respiratory infections (ARI) was launched in April 2025, enabling monitoring of multiple respiratory pathogens. Human parainfluenza virus type 3 (HPIV3) is an important cause of respiratory infections. In 2025, HPIV3 increased from spring to summer, and …

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Establishment of a human coronavirus HKU1 infection evaluation system using apical-out airway organoids

Masatoshi Kakizaki, Satoko Sugimoto, Miyuki Kawase, Sakurako Norito et autres

Human coronavirus HKU1 (HCoV-HKU1) is difficult to study because it does not propagate in standard cell lines. Consequently, research has relied on the air–liquid interface model, which is limited by long culture periods and the absence of cytopathic effects (CPEs). To address …

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Temporal genetic changes in human bocavirus 1 in Fukushima, Japan, from 2018 to 2024

Tatsuya Shirai, Miyuki Kawase, Satoko Sugimoto, Masatoshi Kakizaki et autres

Introduction. Human bocavirus 1 (HBoV1) is a member of Bocaparvovirus primate1 and is associated with paediatric respiratory infections. Although genetic grouping has been described, it remains unclear whether temporal genetic changes occur among clinically circulating HBoV1 strains. Hypothesis/Gap Statement. HBoV1 shows limited …

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Identification of Nectin-4 as a rubella virus entry receptor mediating viral shedding and congenital transmission

Yoshio Mori, Masafumi Sakata, Yuichiro Nakatsu, Takeshi Hori et autres

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Potential for prolonged replication of common acute respiratory viruses in air-liquid interface cultures of primary human airway cells

Miyuki Kawase, Reiko Suwa, Satoko Sugimoto, Masatoshi Kakizaki et autres

A previous study provides clinical evidence that extended respiratory virus detection can occur in healthy pediatric individuals. However, it remains unclear how long respiratory viruses can survive in the human respiratory tissue. In this study, the replication potential of common respiratory viruses …

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Changes in ORF4 of HCoV-229E under different culture conditions

Yuki Kitai, Shohei Kojima, Aikeda Aishajiang, Miyuki Kawase et autres

The genome of human coronavirus 229E (HCoV-229E), a causative agent of human respiratory infections, encodes a unique accessory gene, ORF4. Analysis of laboratory strains and clinical specimens has suggested that HCoV-229E acquires truncating mutations in ORF4 under standard laboratory culture conditions. This …

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A Clinical Isolate of Human Parainfluenza Virus 3 (Fukushima/O695/2019) Contains a Mutation in the Serial Guanosines in the RNA Editing Site of the P Gene and Produces an Atypical Number of Nongenomic Guanosine Insertions During RNA Editing

Kazuya Shirato, Miyuki Kawase, Reiko Suwa, Masatoshi Kakizaki et autres

Human parainfluenza virus 3 (HPIV3) contains a purine-rich RNA editing site, allowing multiple viral proteins to be produced from a single gene by the posttranscriptional addition of G nucleotides. The Fukushima/O695/2019 (O695) HPIV3 clinical isolate has a G-to-A substitution at the last …

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Temporal Trends in Respiratory Infection Epidemics Among Pediatric Inpatients Throughout the Course of the COVID‐19 Pandemic From 2018 to 2023 in Fukushima Prefecture, Japan

Yohei Kume, Koichi Hashimoto, Hisao Okabe, Sakurako Norito et autres

BACKGROUND: Nonpharmaceutical interventions for coronavirus disease (COVID-19), caused by severe acute respiratory syndrome coronavirus 2, during the pandemic altered the epidemiology of respiratory viruses. This study aimed to determine the changes in respiratory viruses among children hospitalized from 2018 to 2023. METHODS: …

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Author response for "Temporal Trends in Respiratory Infection Epidemics Among Pediatric Inpatients Throughout the Course of the COVID‐19 Pandemic From 2018 to 2023 in Fukushima Prefecture, Japan"

Yohei Kume, Koichi Hashimoto, Hisao Okabe, Sakurako Norito et autres

Comparison of mutations in human parainfluenza viruses during passage in primary human bronchial/tracheal epithelial air-liquid interface cultures and cell lines

Satoko Sugimoto, Miyuki Kawase, Reiko Suwa, Yohei Kume et autres

Human parainfluenza virus (HPIV) causes respiratory infections, which are exacerbated in children and older people. Correct evaluation of viral characteristics is essential for the study of countermeasures. However, adaptation of viruses to cultured cells during isolation or propagation might select laboratory passage-associated …

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Molecular Epidemiology of Human Metapneumovirus in East Japan before and after COVID-19, 2017–2022

Kazuya Shirato, Reiko Suwa, Naganori Nao, Miyuki Kawase et autres

Human metapneumovirus (hMPV) is genetically classified into two major subgroups, A and B, based on attachment glycoprotein (G) gene sequences, and the A2 subgroup is further separated into three subdivisions A2a, A2b (A2b1), and A2c (A2b2). The appearance of subgroup A2c viruses …

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Evidence of the simultaneous replications of active viruses in specimens positive for multiple respiratory viruses

Miyuki Kawase, Reiko Suwa, Satoko Sugimoto, Masatoshi Kakizaki et autres

ABSTRACT Genetic diagnostic assays for the detection of respiratory viruses sometimes show simultaneous multiple infections with low copy numbers. In such cases, the disease is considered caused by a single etiologic agent and others are nonspecific reactions and/or contaminations. Interferon-dependent interference is …

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