Andrew N. Hanna

The Use and Utility of Adjuvant Checkpoint Inhibitors in Elderly Patients with Melanoma: A Single Institution Experience

Maira A Bhatty, Natalie N. Chakraborty, Kevin Zablonski, Jarred M. Boone et autres

Background: Elderly patients have low utilization of adjuvant ICIs, although they achieve similar RFS benefits as younger patients. It remains unclear why elderly patients use adjuvant ICIs less frequently and whether toxicity impacts treatment utilization. Methods: Adult patients with stage III melanoma …

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Insights into mRNA lipid nanoparticle polydispersity and shape using quantitative solution biophysics

Marshall S. Padilla, Sarah J. Shepherd, Andrew N. Hanna, Martin Kurnik et autres

Lipid nanoparticles (LNPs) are the most advanced delivery system currently available for RNA therapeutics. Their development has accelerated rapidly since the success of Patisiran, the first siRNA-LNP therapeutic, and the SARS-CoV-2 mRNA vaccines that emerged during the COVID-19 pandemic. Designing LNPs with …

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Multi-stage-mixing to control the supramolecular structure of lipid nanoparticles, thereby creating a core-then-shell arrangement that improves performance by orders of magnitude

Jia Nong, Xijing Gong, Quang Minh Dang, Sachchidanand Tiwari et autres

As they became the dominant gene therapy platform, lipid nanoparticles (LNPs) experienced nearly all their innovation in varying the structure of individual molecules in LNPs. This ignored control of the spatial arrangement of molecules, which is suboptimal because supramolecular structure determines function …

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Figure S3 from Tyrosine Kinase Inhibition Activates Intratumoral γδ T Cells in Gastrointestinal Stromal Tumor

Mark S. Etherington, Andrew N. Hanna, Benjamin D. Medina, Mengyuan Liu et autres

Viability of HG129 cells or T1 GIST cells after 24 hours of (A, B) human IL17a or (C, D) anti-human IL17Ra. Representative of 2 separate experiments with experimental groups in triplicate. Data represent mean ± SEM; p values were calculated using a …

Figure S4 from Tyrosine Kinase Inhibition Activates Intratumoral γδ T Cells in Gastrointestinal Stromal Tumor

Mark S. Etherington, Andrew N. Hanna, Benjamin D. Medina, Mengyuan Liu et autres

(A) Prevalence of IL17A+ γδ T cells in the spleens of KitV558Δ/+ mice after vehicle or imatinib treatment for 1 week. (B) Relative expression of Il6 by PCR of bulk KitV558Δ/+ tumors after 1 week of vehicle or imatinib. (C) Tumor weight …

Figure S4 from Tyrosine Kinase Inhibition Activates Intratumoral γδ T Cells in Gastrointestinal Stromal Tumor

Mark S. Etherington, Andrew N. Hanna, Benjamin D. Medina, Mengyuan Liu et autres

(A) Prevalence of IL17A+ γδ T cells in the spleens of KitV558Δ/+ mice after vehicle or imatinib treatment for 1 week. (B) Relative expression of Il6 by PCR of bulk KitV558Δ/+ tumors after 1 week of vehicle or imatinib. (C) Tumor weight …

Table S2 from Tyrosine Kinase Inhibition Activates Intratumoral γδ T Cells in Gastrointestinal Stromal Tumor

Mark S. Etherington, Andrew N. Hanna, Benjamin D. Medina, Mengyuan Liu et autres

Notable Module 3 genes

Data from Tyrosine Kinase Inhibition Activates Intratumoral γδ T Cells in Gastrointestinal Stromal Tumor

Mark S. Etherington, Andrew N. Hanna, Benjamin D. Medina, Mengyuan Liu et autres

Abstract γδ T cells are a rare but potent subset of T cells with pleiotropic functions. They commonly reside within tumors but the response of γδ T cells to tyrosine kinase inhibition is unknown. To address this, we studied a genetically engineered …

Figure S2 from Tyrosine Kinase Inhibition Activates Intratumoral γδ T Cells in Gastrointestinal Stromal Tumor

Mark S. Etherington, Andrew N. Hanna, Benjamin D. Medina, Mengyuan Liu et autres

Supplemental Figure S2. Relative expression on a per cell basis in KitV558Δ/+ tumors of (A) Trdv4 and (B) multiple genes known to be members of the SCART1+ γδ T cell phenotype. Dot plots represent scRNAseq data of γδ T cells from 6 …

Figure S3 from Tyrosine Kinase Inhibition Activates Intratumoral γδ T Cells in Gastrointestinal Stromal Tumor

Mark S. Etherington, Andrew N. Hanna, Benjamin D. Medina, Mengyuan Liu et autres

Viability of HG129 cells or T1 GIST cells after 24 hours of (A, B) human IL17a or (C, D) anti-human IL17Ra. Representative of 2 separate experiments with experimental groups in triplicate. Data represent mean ± SEM; p values were calculated using a …

Table S1 from Tyrosine Kinase Inhibition Activates Intratumoral γδ T Cells in Gastrointestinal Stromal Tumor

Mark S. Etherington, Andrew N. Hanna, Benjamin D. Medina, Mengyuan Liu et autres

Flow cytometry antibodies

Data from Tyrosine Kinase Inhibition Activates Intratumoral γδ T Cells in Gastrointestinal Stromal Tumor

Mark S. Etherington, Andrew N. Hanna, Benjamin D. Medina, Mengyuan Liu et autres

Abstract γδ T cells are a rare but potent subset of T cells with pleiotropic functions. They commonly reside within tumors but the response of γδ T cells to tyrosine kinase inhibition is unknown. To address this, we studied a genetically engineered …