Scott T. Laughlin

Viscoelastic gel formation in nanoclay-based Pickering emulsions with adsorbing non-ionic polymers

Harshika N. Disanayaka, Nipuni Gunawardhana, Scott T. Laughlin, Surita R. Bhatia

The stabilization of Pickering emulsions by nanoclays and water-soluble polymers presents a promising strategy for altering emulsion structure and rheology. In this study, the influence of poly(ethylene oxide) (PEO) with a molecular weight of approximately 100 kg mol −1 on the rheology …

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Exploring synthetic routes to 6-functionalized 4-azaspiro[2.3]hexanes

Wei Huang, K.-C. Wen, Scott T. Laughlin

Azaspirohexanes are promising scaffolds for the synthesis of piperidine bioisosteres in medicinal chemistry. Previous work in this area has focused on developing 5-azaspiro[2.3]hexane analogues, but the synthesis of 6-functionalized 4-azaspiro[2.3]hexanes remains largely unexplored. To synthesize 6-functionalized 4-azaspiro[2.3]hexane analogues, we explored the use …

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Chemogenetic and optogenetic strategies for spatiotemporal control of split-enzyme-based calcium recording

Yunlei Zhang, Brianna K. Campmier, Kelly N. Eckartt, Scott T. Laughlin

ABSTRACT Methods for monitoring physiological changes in cellular Ca 2+ levels have been in high demand for their utility in monitoring neuronal signaling. Recently, we introduced SCANR (Split-Tobacco Etch Virus (TEV) protease Calcium-regulated Neuron Recorder), which reports on Ca 2+ changes in …

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Blood-brain-barrier permeable fluorescent astrocyte probes

Nipuni Gunawardhana, Danielle A. Cervasio, Oshini Haputhanthrige-Don, Scott T. Laughlin

The brain's astrocytes play pivotal roles in brain homeostasis, but their contributions to neural circuit function are only beginning to be explored. In order to study astrocytes in their native environment, the field needs imaging probes that are cell-specific, non-toxic, and complementary …

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Modular caged chlorooximes for cell-selective bioorthogonal labeling

Wei Huang, Yunlei Zhang, K.-C. Wen, Scott T. Laughlin

Conventional bioorthogonal reactions label biomolecules indiscriminately across the entire cell or organism, which can produce unwanted background signal that complicates analyses of specific cell types, tissues, or disease states. Activatable bioorthogonal reactions have been developed to address this limitation. These reactions enable …

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Evaluation of Pyrones in Bioorthogonal Reactions: Correlation between Structure, Reactivity, and Bioorthogonality

Wei Huang, K.-C. Wen, Paul F. Muschitiello, Jorge Escorihuela et autres

Alpha-pyrones have been used for applications ranging from total synthesis to antibiotics. However, their application as dienes in bioorthogonal reactions has not been extensively explored. In previous work, we demonstrated the promising application of ester-functionalized pyrones in bioorthogonal protein labeling. Here, we …

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Click Chemistry for Cell Labeling

Wei Huang, Wei‐Siang Kao, Scott T. Laughlin

Click chemistry has advanced over the past two decades to enable the imaging of diverse biomolecules in cells, including non-genetically encoded molecules like lipids and glycans. In the toolbox of bioorthogonal click reactions for cell labeling, pro-fluorescent tetrazines stand out owing to …

Author response for "Unveiling the Reactivity of 2H‑(Thio)pyran-2-(thi)ones in Cycloaddition Reactions with Strained Alkynes through Density Functional Theory Studies"

Wei Huang, K.-C. Wen, Scott T. Laughlin, Jorge Escorihuela

Redox‐Activated Substrates for Enhancing Activatable Cyclopropene Bioorthogonal Reactions

Wei‐Siang Kao, Wei Huang, Yunlei Zhang, K.-C. Wen et autres

Bioorthogonal chemistry has become a mainstay in chemical biology and is making inroads in the clinic with recent advances in protein targeting and drug release. Since the field's beginning, a major focus has been on designing bioorthogonal reagents with good selectivity, reactivity, …

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Unveiling the reactivity of 2 H -(thio)pyran-2-(thi)ones in cycloaddition reactions with strained alkynes through density functional theory studies

Wei Huang, K.-C. Wen, Scott T. Laughlin, Jorge Escorihuela

A theoretical study of the IEDDA reaction of endo -BCN with 2 H -pyran-2-one, 2 H -thiopyran-2-one, 2 H -pyran-2-thione and 2 H -thiopyran-2-thione reveals the superior reactivity of 2 H -pyran-2-thiones because of the lower distortion energy.

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Pyranthiones/Pyrones: “Click and Release” Donors for Subcellular Hydrogen Sulfide Delivery and Labeling**

Wei Huang, Nipuni Gunawardhana, Yunlei Zhang, Jorge Escorihuela et autres

Abstract Hydrogen sulfide (H 2 S), one of the most important gasotransmitters, plays a critical role in endogenous signaling pathways of many diseases. However, developing H 2 S donors with both tunable release kinetics and high release efficiency for subcellular delivery has …

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Cell-selective bioorthogonal labeling

Wei Huang, Scott T. Laughlin

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