Click Chemistry for Cell Labeling

Le résumé fourni par la source

Click chemistry has advanced over the past two decades to enable the imaging of diverse biomolecules in cells, including non-genetically encoded molecules like lipids and glycans. In the toolbox of bioorthogonal click reactions for cell labeling, pro-fluorescent tetrazines stand out owing to their fast kinetics and low background fluorescence when reacting with dienophiles like trans-cyclooctene, bicyclononynes, or cyclopropenes. In this chapter, we present a brief overview of click chemistry approaches to cell labeling followed by an in-depth review of pro-fluorescent tetrazines. We review key properties for cell imaging, including ligation rates, fluorescence turn-on ratios and excitation/emission spectra. Finally, we discuss recent applications in biology for click chemistry-based cell imaging based on fluorogenic tetrazines and on activatable tetrazine ligations for cell-selective labeling.

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