Olaf Bergmann

All-optical closed-loop control of human cardiomyocyte networks exploiting holographic optogenetics

Robert Wendland, Felix Schmieder, Muhammad Atif Sikandar, Frithjof Paul Knüppel et autres

Heart diseases can trigger life-threatening arrhythmias by disrupting cardiac contraction wavefront propagation. In contrast to conventional treatments like electrical pacemakers, optogenetics offers high spatial and temporal resolution for non-invasive interrogation and modulation of cellular activity. However, combining sensing and actuation into a …

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Real-time optogenetic control of excitation wavefronts in human cardiomyocytes using label-free and sparse sensing

Robert Wendland, Felix Schmieder, Muhammad Atif Sikandar, Wolfram‐Hubertus Zimmermann et autres

Cardiac diseases are characterized by deviations from normal spatiotemporal contraction wavefront patterns. Optogenetics offers ways to investigate and mitigate these states. System integration of optogenetic control strategies requires real-time control of cardiac contractions but is often hindered by the data processing load …

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Real-time optogenetic control of excitation wavefronts in human cardiomyocytes using holographic one- and two-photon stimulation

Robert Wendland, Felix Schmieder, Muhammad Sikandar, Wolfram Hubertus Zimmermann et autres

An all-optical method to monitor, stimulate and feedback-control excitation wavefronts in human cardiomyocyte networks in real-time is presented. We employ ferro-electric spatial light modulators for fast (~kHz) display of excitation patterns and sparsely sampled optical signals to characterize the prevalent state of …

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Left Ventricular Assist Device–Mediated Unloading and Circulatory Support as a Potential Regenerative Strategy in Advanced Heart Failure

Olaf Bergmann, Eleni Tseliou, Hesham A. Sadek, Stavros G. Drakos

http://dx.doi.org/10.13039/501100004359 Swedish Research Council

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Label-free microscopy for optogenetic investigations of arrhythmia in human cardiomyocyte networks expressing Chrimson

Robert Wendland, Felix Schmieder, Muhammad Suleman Sikandar, Wolfram‐Hubertus Zimmermann et autres

Spatiotemporal gene expression and cellular dynamics of the developing human heart

Enikő Lázár, Raphaël Mauron, Žaneta Andrusivová, Julia Foyer et autres

Heart development relies on topologically orchestrated cellular transitions and interactions, many of which remain poorly characterized in humans. Here, we combined unbiased spatial and single-cell transcriptomics with imaging-based validation across postconceptional weeks 5.5 to 14 to uncover the molecular landscape of human …

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Excitation wavefront tracking and control of in vitro human induced cardiomyocytes using a digital holographic stimulation system

Felix Schmieder, Robert Wendland, Wolfram‐Hubertus Zimmermann, Lars Büttner et autres

Dysfunctional signal propagation patterns in hearts like self-replicating reentrant spiral waves are a main cause of tachycardia, whose origin is not yet fully understood. We aim to investigate related phenomena using optogenetics to overcome spatiotemporal resolution restrictions of electrical or chemical stimulation …

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Label-Free Sensing and Real-Time Holographic Optogenetic Control of Cardiac Excitation Wavefronts

Lars Büttner, Robert Wendland, Felix Schmieder, Muhammad A. Sikandar et autres

We present label-free real-time cardiac excitation wavefront characterization to identify pathologic contraction states. Sparsely sampled optical signals are used for optogenetic closed-loop wavefront control of in vitro human cardiomyocyte networks expressing Chrimson.

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A Latent Cardiomyocyte Regeneration Potential in Human Heart Disease

Wouter J. A. Derks, Julian Rode, Sofia Collin, Fabian Rost et autres

BACKGROUND: Cardiomyocytes in the adult human heart show a regenerative capacity, with an annual renewal rate of ≈0.5%. Whether this regenerative capacity of human cardiomyocytes is employed in heart failure has been controversial. METHODS: We determined cardiomyocyte renewal in 52 patients with …

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Editorial: Methods in cardiovascular biologics and regenerative medicine

Narasimman Gurusamy, Olaf Bergmann, Clotilde Castaldo, Ngan Fong Huang et autres

Cardiovascular diseases remain the leading cause of mortality globally, accounting for 20.5 million deaths in 2021, which is approximately one-third of all deaths annually (1).Given the complexity and substantial economic burden posed by cardiovascular risk factors and diseases (2,3), it is imperative …

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Spatially resolved multiomics on the neuronal effects induced by spaceflight in mice

Yuvarani Masarapu, Egle Cekanaviciute, Žaneta Andrusivová, Jakub Orzechowski Westholm et autres

Impairment of the central nervous system (CNS) poses a significant health risk for astronauts during long-duration space missions. In this study, we employed an innovative approach by integrating single-cell multiomics (transcriptomics and chromatin accessibility) with spatial transcriptomics to elucidate the impact of …

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Thioredoxin-2 protects mice against experimental myocardial infarction

Tania Medali, Dominique Couchie, Nathalie Mougenot, Maria Mihoc et autres

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