Rodrigo Coutinho de Almeida

Metagenomics in Spaceflight: Establishing an Implementation Roadmap

Jared T. Broddrick, Jonathan E. Allen, Jyothi Basapathi Raghavendra, Christopher E. Carr et autres

As space agencies prepare for complex missions such as human exploration of Mars and life detection, Planetary Protection (PP) risk assessments would benefit from extending beyond traditional microbial monitoring methods like the Standard Spore Assay (SSA). Metagenomics offers a powerful complementary approach, …

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Building AI-Ready Data Systems for Space Life Sciences, Aerospace Medicine, and Deep Space Exploration

Sylvain V. Costes, Sergio Garcia Busto, Ryan T. Scott, James A. Casaletto et autres

While AI holds the potential to revolutionize space life sciences, realizing this promise is contingent upon the systematic restructuring of heterogeneous spaceflight biological data into machine-actionable, AI-ready forms. Even though open access principles support human reuse and scientific reproducibility, this does not …

Building AI-Ready Data Systems for Space Life Sciences, Aerospace Medicine, and Deep Space Exploration

Sylvain V. Costes, Sergio Garcia Busto, Ryan T. Scott, James A. Casaletto et autres

While AI holds the potential to revolutionize space life sciences, realizing this promise is contingent upon the systematic restructuring of heterogeneous spaceflight biological data into machine-actionable, AI-ready forms. Even though open access principles support human reuse and scientific reproducibility, this does not …

us, fr, nl, se, gb (code pays fourni par la source)

ExocubeBio: an in-situ fluidic platform for microbial exposure on the International Space Station

David J. Burr, Ruben Nitsche, Elisa Ravaro, Severin Wipf et autres

Abstract Space-based platforms currently represent the most accurate means to experimentally assess the influence of the space environment on biological systems. However, performing such experiments remains technically challenging and requires highly specialized instrumentation. This study describes the current development and hardware qualification …

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Associations of healthy lifestyle and genetic susceptibility with risks of osteoarthritis: a prospective cohort study

Shibo Chen, Yujie Zhang, Tianxiang Fan, Muhui Zeng et autres

OBJECTIVES: To detect the associations of a healthy lifestyle and genetic susceptibility with incident OA in the UK Biobank study. METHODS: We included 314 729 participants from UK Biobank. Five modifiable lifestyle factors including weight management, diet, physical activity, sleep behaviour and …

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Resolving Temporally Ambiguous Relationships between Single-Nucleus Multimodal Sequencing Derived Cell Populations

Rick Mulders, Mathew Nickel Maunu, Rodrigo Coutinho de Almeida, Marcella van Hoolwerff et autres

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The role of DNA methylation in chondrogenesis of human iPSCs as a stable marker of cartilage quality

Ghazaleh Hajmousa, Rodrigo Coutinho de Almeida, Niek G.C. Bloks, Alejandro Rodríguez Ruiz et autres

BACKGROUND: Lack of insight into factors that determine purity and quality of human iPSC (hiPSC)-derived neo-cartilage precludes applications of this powerful technology toward regenerative solutions in the clinical setting. Here, we set out to generate methylome-wide landscapes of hiPSC-derived neo-cartilages from different …

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Dissecting SOX9 dynamics reveals its differential regulation in osteoarthritis

Kannan Govindaraj, Sakshi Kannan, Rodrigo Coutinho de Almeida, Lucas Jansen Klomp et autres

The transcription factor SOX9 is integral to tissue homeostasis and is implicated in skeletal malformation, campomelic dysplasia, and osteoarthritis (OA). Despite extensive research, the complete regulatory landscape of SOX9 transcriptional activity, interconnected with signaling pathways (TGFβ, WNT, BMP, IHH, NFκB, and HIF), …

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A Damaging COL6A3 Variant Alters the MIR31HG ‐Regulated Response of Chondrocytes in Neocartilage Organoids to Hyperphysiologic Mechanical Loading

Niek G.C. Bloks, Zainab Harissa, Giorgia Mazzini, Shaunak Sanjay Adkar et autres

The pericellular matrix (PCM), with its hallmark proteins collagen type VI (COLVI) and fibronectin (FN), surrounds chondrocytes and is critical in transducing the biomechanical cues. To identify genetic variants that change protein function, exome sequencing is performed in a patient with symptomatic …

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The potential of five c-miRNAs as serum biomarkers for Late-Onset Alzheimer’s disease diagnosis: miR-10a-5p, miR-29b-2-5p, miR-125a-5p, miR-342-3p, and miR-708-5p

Isadora de Lourdes Signorini-Souza, Luciane Viater Tureck, Meire Silva Batistela, Rodrigo Coutinho de Almeida et autres

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Hyper-physiologic mechanical cues, as an osteoarthritis disease-relevant environmental perturbation, cause a critical shift in set points of methylation at transcriptionally active CpG sites in neo-cartilage organoids

Niek G.C. Bloks, Amanda R. Dicks, Zainab Harissa, Rob G. H. H. Nelissen et autres

BACKGROUND: Osteoarthritis (OA) is a complex, age-related multifactorial degenerative disease of diarthrodial joints marked by impaired mobility, joint stiffness, pain, and a significant decrease in quality of life. Among other risk factors, such as genetics and age, hyper-physiological mechanical cues are known …

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Thyroid hormone induces ossification and terminal maturation in a preserved OA cartilage biomimetic model

Nicoline M. Korthagen, Evelyn Houtman, Irene U. Boone, Rodrigo Coutinho de Almeida et autres

OBJECTIVE: To characterize aspects of triiodothyronine (T3) induced chondrocyte terminal maturation within the molecular osteoarthritis pathophysiology using the previously established T3 human ex vivo osteochondral explant model. DESIGNS: RNA-sequencing was performed on explant cartilage obtained from OA patients (n = 8), that …

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