Rainer Schneider

Python-based Pulseq Client to the Remote Sequence Streaming Interface

Mojtaba Shafiekhani, T. Kluge, Ben Wilhelm-Feldbusch, Patrick Hucker et autres

Motivation: Tools for cross-platform open-source pulse sequence development have recently gained substantial traction within the MRI community. An important ingredient thereof is the set of scanner-specific "driver" or "interpreter" software modules, which currently have to be compiled and tested for every scanner …

Scar-free tag removal by CASPON® enzyme with broad physicochemical stability in biomanufacturing – A case study of five proteins

Daniel J. Elsner, Christoph Köppl, Maximilian Kogler, Patrick Scheidl et autres

A comprehensive study of SARS-CoV-2 main protease (Mpro) inhibitor-resistant mutants selected in a VSV-based system

Francesco Costacurta, Andrea Dodaro, David Bante, Helge Schöppe et autres

Nirmatrelvir was the first protease inhibitor specifically developed against the SARS-CoV-2 main protease (3CLpro/Mpro) and licensed for clinical use. As SARS-CoV-2 continues to spread, variants resistant to nirmatrelvir and other currently available treatments are likely to arise. This study aimed to identify …

at, it, us, sg, sa (code pays fourni par la source)

Premature cognitive decline in a mouse model of tuberous sclerosis

Jennifer Krummeich, Laura De Nardi, Cosima Caliendo, Dominik F. Aschauer et autres

Abstract Little is known about the influence of (impaired) neurodevelopment on cognitive aging. We here used a mouse model for tuberous sclerosis (TS) carrying a heterozygous deletion of the Tsc2 gene. Loss of Tsc2 function leads to mTOR hyperactivity in mice and …

de, at (code pays fourni par la source)

Skeletal muscle proteome analysis underpins multifaceted mitochondrial dysfunction in Friedreich’s ataxia

Elisabetta Indelicato, Klaus Faserl, Matthias Amprosi, Wolfgang Nachbauer et autres

Friedreich’s ataxia (FRDA) is a severe multisystemic disorder caused by a deficiency of the mitochondrial protein frataxin. While some aspects of FRDA pathology are developmental, the causes underlying the steady progression are unclear. The inaccessibility of key affected tissues to sampling is …

at (code pays fourni par la source)

A comprehensive study of SARS-CoV-2 main protease (M pro ) inhibitor-resistant mutants selected in a VSV-based system

Francesco Costacurta, Andrea Dodaro, David Bante, Helge Schöppe et autres

Abstract Nirmatrelvir was the first protease inhibitor (PI) specifically developed against the SARS-CoV-2 main protease (3CL pro /M pro ) and licensed for clinical use. As SARS-CoV-2 continues to spread, variants resistant to nirmatrelvir and other currently available treatments are likely to …

at, it, us, ru, sg (code pays fourni par la source)

A comprehensive study of SARS-CoV-2 main protease (M pro ) inhibitor-resistant mutants selected in a VSV-based system

Francesco Costacurta, Andrea Dodaro, David Bante, Helge Schöppe et autres

Topology, trajectory and config files for each case presented in the publication.

CASPON technology – A platform process for non‐platform proteins using Escherichia coli

Monika Cserjan‐Puschmann, Christoph Köppl, Nico Lingg, Rainer Schneider et autres

at (code pays fourni par la source)

CASPON platform technology: Ultrafast circularly permuted caspase-2 cleaves tagged fusion proteins before all 20 natural amino acids at the N-terminus

Nico Lingg, Christina Kröß, Petra Engele, Christoph Öhlknecht et autres

Fusion protein technologies improve the expression and purification of recombinant proteins, but the removal of the tags involved requires specific proteases. The circularly permuted caspase-2 (cpCasp2) with its specific cleavage site, efficiently generates the untagged protein. While cleavage with cpCasp2 is possible …

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Fusion Tag Design Influences Soluble Recombinant Protein Production in Escherichia coli

Christoph Köppl, Nico Lingg, Andreas M. Fischer, Christina Kröß et autres

are widely used but may also be associated with negative consequences. Although commonly employed solubility tags have a positive influence on titers, their large molecular mass inherently results in stochiometric losses of product yield. Furthermore, the introduction of affinity tags, especially the …

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PROFICS: A bacterial selection system for directed evolution of proteases

Christina Kröß, Petra Engele, Bernhard Sprenger, Andreas M. Fischer et autres

Proteases serve as important tools in biotechnology and as valuable drugs or drug targets. Efficient protein engineering methods to study and modulate protease properties are thus of great interest for a plethora of applications. We established PROFICS (PRotease Optimization via Fusion-Inhibited Carbamoyltransferase-based …

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The Diarylheptanoid Curcumin Induces MYC Inhibition and Cross-Links This Oncoprotein to the Coactivator TRRAP

Alexander Mödlhammer, Sandra Pfurtscheller, Andreas Feichtner, Markus Hartl et autres

The c-Myc protein (MYC) is a transcription factor with strong oncogenic potential controlling fundamental cellular processes. In most human tumors, MYC is overexpressed by enhanced transcriptional activation, gene amplification, chromosomal rearrangements, or increased protein stabilization. To pharmacologically suppress oncogenic MYC functions, multiple …

at (code pays fourni par la source)