Jesus Magallón

Full Validation of Pooled Antibiotic Susceptibility Testing Using CLSI Methods and Performance Criteria in UTI Pathogens

Richard A. Festa, F. R. Cockerill, Rick L. Pesano, Emery Haley et autres

Background: Here, we validate a unique and rapid susceptibility assay, Pooled Antibiotic Susceptibility Testing (P-AST), used for complicated, persistent, and recurrent urinary tract infections (UTIs), following Clinical and Laboratory Standards Institute (CLSI) protocols and performance metrics. Methods: P-AST™ was validated against the …

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Pooled Antibiotic Susceptibility Testing for Polymicrobial UTI Performs Within CLSI Validation Standards

Richard A. Festa, F. R. Cockerill, Rick L. Pesano, Emery Haley et autres

BACKGROUND/OBJECTIVES: Urinary tract infections (UTIs) pose an increasing risk of antimicrobial resistance, and novel diagnostic tests have been developed to address the limitations of standard urine culture in these cases. It is important that these novel tests be validated for agreement and …

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Urine biomarkers individually and as a consensus model show high sensitivity and specificity for detecting UTIs

Marzieh Akhlaghpour, Emery Haley, Laura K. S. Parnell, Natalie Luke et autres

BACKGROUND: Current diagnoses of urinary tract infection (UTI) by standard urine culture (SUC) has significant limitations in sensitivity, especially for fastidious organisms, and the ability to identify organisms in polymicrobial infections. The significant rate of both SUC "negative" or "mixed flora/contamination" results …

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Quantitative multiplex polymerase chain reaction in copies ml–1 linearly correlates with standard urine culture in colonies ml−1 for urinary tract infection (UTI) pathogens

Richard A. Festa, Mike Opel, Mohit Mathur, Natalie Luke et autres

Standard urine culture (SUC) is the current standard method for confirmation of a urinary tract infection (UTI). SUC identifies microorganisms in urine samples and semi-quantifies these as colony-forming units (CFUs) ml-1. In contrast, quantitative multiplex polymerase chain reaction (q-MPCR) is a culture-independent …

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Urine Biomarkers Individually and as a Consensus Model Show High Sensitivity and Specificity for Detecting UTIs

Marzieh Akhlaghpour, Emery Haley, Laura K. S. Parnell, Natalie Luke et autres

We aimed to determine if infection-associated urine biomarkers can differentiate true urinary tract infection (UTI) from non-UTI controls. Midstream clean-catch urine samples were collected from asymptomatic volunteers and symptomatic subjects > 60 years old diagnosed with presumptive UTI in a specialty setting. …

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A test combining multiplex-PCR with pooled antibiotic susceptibility testing has high correlation with expanded urine culture for detection of live bacteria in urine samples of suspected UTI patients

Richard A. Festa, Natalie Luke, Mohit Mathur, Laura K. S. Parnell et autres

We evaluated whether multiplex polymerase chain reaction (M-PCR) detects viable micro-organisms by comparing micro-organism identification with standard urine culture (SUC) and expanded quantitative urine culture (EQUC). Of the 395 organisms detected by M-PCR, EQUC detected 89.1% (p = 0.10), whereas SUC detected …

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A Test Combining Multiplex-PCR with Pooled Antibiotic Susceptibility Testing Has High Correlation with Expanded Urine Culture for Detection of Live Bacteria in Urine Samples of Suspected UTI Patients

Richard A. Festa, Natalie Luke, Mohit Mathur, Laura K. S. Parnell et autres

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Amikacin potentiator activity of zinc complexed to a pyrithione derivative with enhanced solubility

Jesus Magallón, Peter Vu, Craig Reeves, Stella Kwan et autres

Abstract Resistance to amikacin in Gram-negatives is usually mediated by the 6'- N -acetyltransferase type Ib [AAC(6')-Ib], which catalyzes the transfer of an acetyl group from acetyl CoA to the 6' position of the antibiotic molecule. A path to continue the effective …

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Amikacin potentiator activity of zinc complexed to a pyrithione derivative with enhanced solubility

Jesus Magallón, Peter Vu, Craig Reeves, Stella Kwan et autres

Resistance to amikacin in Gram-negatives is usually mediated by the 6′- N -acetyltransferase type Ib [AAC(6′)-Ib], which catalyzes the transfer of an acetyl group from acetyl CoA to the 6′ position of the antibiotic molecule. A path to continue the effective use …

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Amikacin in combination with zinc pyrithione prevents growth of a multidrug-resistant carbapenem-resistant Klebsiella pneumoniae isolate

Jesus Magallón, Peter Vu, Craig Reeves, Stella Kwan et autres

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Inhibition of Aminoglycoside 6′-N-acetyltransferase Type Ib (AAC(6′)-Ib): Structure–Activity Relationship of Substituted Pyrrolidine Pentamine Derivatives as Inhibitors

Kenneth Rocha, Jesus Magallón, Craig Reeves, Kimberly Phan et autres

The aminoglycoside 6′-N-acetyltransferase type Ib (AAC(6′)-Ib) is a common cause of resistance to amikacin and other aminoglycosides in Gram-negatives. Utilization of mixture-based combinatorial libraries and application of the positional scanning strategy identified an inhibitor of AAC(6′)-Ib. This inhibitor’s chemical structure consists of …

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Inhibition of Aminoglycoside 6’-N-Acetyltransferase Type Ib [Aac(6′)-Ib]: Structure-Activity Relationship of Substituted Pyrrolidine Pentamine Derivatives as Inhibitors

Kenneth Rocha, Jesus Magallón, Craig Reeves, Kimberly Phan et autres

The aminoglycoside 6′-N-acetyltransferase type Ib [AAC(6′)-Ib] is a common cause of resistance to amikacin and other aminoglycosides in Gram-negatives. Utilization of mixture-based combinatorial libraries and application of the positional scanning strategy identified an inhibitor of AAC(6′)-Ib. This inhibitor’s chemical structure consists of …

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