Shannon E. McGettigan

Supplemental Figure 1 from CAR Binders Affect CAR T-cell Tonic Signaling, Durability, and Sensitivity to Target

Deepa Shukla, Khatuna Gabunia, Shannon E. McGettigan, Prachi R. Patel et autres

Supplemental Figure 1: a-b) Primary human T cells stimulated using anti-CD3/28 beads and on the following day were transduced with indicated CARs. Cells were stained with G4S linker antibody (a) and FITC-labeled human CD19 protein (b). c) After 12 days of culture, …

Supplementary Data from CAR Binders Affect CAR T-cell Tonic Signaling, Durability, and Sensitivity to Target

Deepa Shukla, Khatuna Gabunia, Shannon E. McGettigan, Prachi R. Patel et autres

Table S1

Supplemental Figure 4 from CAR Binders Affect CAR T-cell Tonic Signaling, Durability, and Sensitivity to Target

Deepa Shukla, Khatuna Gabunia, Shannon E. McGettigan, Prachi R. Patel et autres

Supplemental Figure 4: In the validation titration experiment, the isotype control article was screened for binding against targets identified on the MPA screen as well as the positive controls (Protein A and a known binder CD19), and the negative control (pUC empty …

Data from CAR Binders Affect CAR T-cell Tonic Signaling, Durability, and Sensitivity to Target

Deepa Shukla, Khatuna Gabunia, Shannon E. McGettigan, Prachi R. Patel et autres

Abstract Patients can develop human anti-mouse immune responses against CD19-specific chimeric antigen receptor (CAR) T cells due to the use of a murine CD19-specific single-chain variable fragment to redirect T cells. We screened a yeast display library to identify an array of …

Supplementary Data from CAR Binders Affect CAR T-cell Tonic Signaling, Durability, and Sensitivity to Target

Deepa Shukla, Khatuna Gabunia, Shannon E. McGettigan, Prachi R. Patel et autres

Table S2

Supplemental Figure 3 from CAR Binders Affect CAR T-cell Tonic Signaling, Durability, and Sensitivity to Target

Deepa Shukla, Khatuna Gabunia, Shannon E. McGettigan, Prachi R. Patel et autres

Supplemental Figure 3: Anti-CD19 control MAbs HIB19 and CB19 were screened on a CD19 alanine-scan mutation library expressed in HEK 293T cells, with binding assayed by flow cytometry. Binding values are shown as a percentage of the antibodies’ binding to the parental …

Supplemental Figure 2 from CAR Binders Affect CAR T-cell Tonic Signaling, Durability, and Sensitivity to Target

Deepa Shukla, Khatuna Gabunia, Shannon E. McGettigan, Prachi R. Patel et autres

Supplemental Figure 2: a) Survival curve of mice after CART cell treatment. b-d) Peripheral blood was collected from humanized mice on the indicated days after CART treatment and stained with anti-human CD45, CD3, CD4, CD8 antibodies, and CD19 protein. As indicated, mice …

CAR Binders Affect CAR T-cell Tonic Signaling, Durability, and Sensitivity to Target

Deepa Shukla, Khatuna Gabunia, Shannon E. McGettigan, Prachi R. Patel et autres

Patients can develop human anti-mouse immune responses against CD19-specific chimeric antigen receptor (CAR) T cells due to the use of a murine CD19-specific single-chain variable fragment to redirect T cells. We screened a yeast display library to identify an array of fully …

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Abstract 4806: CAR binders affect CAR T cell tonic signaling, durability, and sensitivity to target

Divanshu Shukla, Khatuna Gabunia, Shannon E. McGettigan, Prachi Patel et autres

Patients can develop human anti mouse immune responses against CD19- specific CAR T cells (CARTs) due to the use of a murine scFv to redirect T cells. To mitigate immunogenicity, a humanization process of the FMC63- based binder was undertaken that resulted …

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Secreted IgM modulates IL-10 expression in B cells

Shannon E. McGettigan, Lazaro E. Aira, Gaurav Kumar, Romain Ballet et autres

Abstract IL-10 + B cells are critical for immune homeostasis and restraining immune responses in infection, cancer, and inflammation; however, the signals that govern IL-10 + B cell differentiation are ill-defined. Here we find that IL-10 + B cells expand in mice …

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Supplemental Information and Legends from Safety and Efficacy of Intratumoral Injections of Chimeric Antigen Receptor (CAR) T Cells in Metastatic Breast Cancer

Julia Tchou, Yangbing Zhao, Bruce L. Levine, Paul J. Zhang et autres

Supplemental Information and Legends

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Supplemental Table S2 from Safety and Efficacy of Intratumoral Injections of Chimeric Antigen Receptor (CAR) T Cells in Metastatic Breast Cancer

Julia Tchou, Yangbing Zhao, Bruce L. Levine, Paul J. Zhang et autres

S2. Comprehensive list of all adverse events based on CTCAE v4.0 grading from Day -2 (IT injection) to Day +25.