Simran Kaushal

Transferability and Reproducibility of the HepaRG CometChip Assay

Leslie Recio, Carol D. Swartz, Lincoln Martin, Emily Rottinger et autres

This interlaboratory evaluation of HepaRG CometChip was conducted to assess transferability and reproducibility of this new approach methodology (NAM) across four laboratories. Concentrations inducing up to ~70% relative cytotoxicity were determined by the organizing laboratory, and frozen chemical formulation blocks were sent …

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CometChip enables parallel analysis of multiple DNA repair activities [DNA repair 106 (2021) 103176–103202]

Jing Ge, Le P. Ngo, Simran Kaushal, Ian J. Tay et autres

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Frailty, sex, and poverty are associated with DNA damage and repair in frail, middle-aged urban adults

Jessica Smith, Nicole Noren Hooten, Nicolle A. Mode, Alan B. Zonderman et autres

Frailty is an age-related syndrome characterized by reduced recovery from stressors and increased risks of morbidity and mortality. Although frailty is usually studied in those over 65 years, our previous work showed that frailty is both present and a risk factor for …

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Abstract 4332: Characterizing cancer-associated fibroblasts in prostate cancer using Vizgen’s MERSCOPETM Platform

Ben Patterson, Cheng‐Yi Chen, Nicolás Fernández, Simran Kaushal et autres

Abstract Understanding the spatial complexities within cancer provides crucial information for evaluating individual tumor development and progression; recent research in prostate cancer has shown how critical it is for oncologists to maintain a spatial context while characterizing a highly heterogeneous tumor microenvironment. …

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Using the HepaCometChip Assay for Broad‐Spectrum DNA Damage Analysis

Norah A. Owiti, Simran Kaushal, Lincoln Martin, Jamie E. Sly et autres

Exposure to DNA damaging agents can lead to mutations that cause cancer. The liver is particularly vulnerable because it contains high levels of Cytochrome P450 enzymes that can convert xenobiotics into DNA reactive metabolites that form potentially carcinogenic bulky DNA adducts. As …

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A novel application of acrylic resin UV-Curing methodology for Radioactive surface Decontamination

Simran Kaushal, Sibi Oommen, Shivanand Bhushan

Abstract The facilities dealing with radiation technology face the brunt of Radioactive decontamination. A unique UV-Curing method is provided for decontamination of radioisotopes on the surfaces which has radiation dealing facilities. In this method acrylic UV-Curing resin was poured on a contaminated …

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The Parallel Transformations of Polycyclic Aromatic Hydrocarbons in the Body and in the Atmosphere

Amy Hrdina, Ishwar N. Kohale, Simran Kaushal, Jamie M. Kelly et autres

BACKGROUND: Polycyclic aromatic hydrocarbons (PAHs) emitted from combustion sources are known to be mutagenic, with more potent species also being carcinogenic. Previous studies show that PAHs can undergo complex transformations both in the body and in the atmosphere, yet these transformation processes …

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MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum

Aoli Xiong, Simran Kaushal, Ian Junjie Tay, Bevin P. Engelward et autres

For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020).

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CometChip analysis of human primary lymphocytes enables quantification of inter-individual differences in the kinetics of repair of oxidative DNA damage

Le P. Ngo, Simran Kaushal, Isaac A. Chaim, Patrizia Mazzucato et autres

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CometChip enables parallel analysis of multiple DNA repair activities

Jing Ge, Le P. Ngo, Simran Kaushal, Ian J. Tay et autres

DNA damage can be cytotoxic and mutagenic, and it is directly linked to aging, cancer, and other diseases. To counteract the deleterious effects of DNA damage, cells have evolved highly conserved DNA repair pathways. Many commonly used DNA repair assays are relatively …

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CometChip Enables Parallel Analysis of Multiple DNA Repair Activities

Jing Ge, Le P. Ngo, Simran Kaushal, Ian J. Tay et autres

ABSTRACT DNA damage can be cytotoxic and mutagenic and is directly linked to aging, cancer, and heritable diseases. To counteract the deleterious effects of DNA damage, cells have evolved highly conserved DNA repair pathways. Many commonly used DNA repair assays are relatively …

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Sequence and Nuclease Requirements for Breakage and Healing of a Structure-Forming (AT)n Sequence within Fragile Site FRA16D

Simran Kaushal, Charles Wollmuth, Kohal Das, Suzanne E. Hile et autres

Common fragile sites (CFSs) are genomic regions that display gaps and breaks in human metaphase chromosomes under replication stress and are often deleted in cancer cells. We studied an ∼300-bp subregion (Flex1) of human CFS FRA16D in yeast and found that it …

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