Dorien A. Roosen

A Silicon Rhodamine‐fused Glibenclamide to Label and Detect Malaria‐infected Red Blood Cells

Claudia Bastl, Cindy M. Close, Ingo Holtz, Blaise Gatin‐Fraudet et autres

The malaria parasite Plasmodium falciparum affects the lives of millions of people worldwide every year. The detection of replicating parasites within human red blood cells is of paramount importance, requiring appropriate diagnostic tools. Herein, we design and apply a silicon rhodamine-fused glibenclamide …

de, gb, Nigéria, ch, us (code pays fourni par la source)

A silicon rhodamine-fused glibenclamide to label and detect malaria-infected red blood cells

Claudia Bastl, Cindy M. Close, Ingo Holtz, Blaise Gatin‐Fraudet et autres

Abstract The malaria parasite Plasmodium falciparum affects the lives of millions of people worldwide every year. The detection of replicating parasites within human red blood cells is of paramount importance, requiring appropriate diagnostic tools. Herein, we design and apply a silicon rhodamine-fused …

de, gb, ch, Nigéria, us (code pays fourni par la source)

Sulfonated red and far-red rhodamines to visualize SNAP- and Halo-tagged cell surface proteins

Ramona Birke, Julia Ast, Dorien A. Roosen, Joon Lee et autres

self-labelling protein tags. We anticipate that Sulfo549, Sulfo646 and their congeners will be useful for a number of cell biology applications where labelling of intracellular sites interferes with accurate surface protein analysis.

de, gb, us (code pays fourni par la source)

Transcriptional signatures in iPSC-derived neurons are reproducible across labs when differentiation protocols are closely matched

Xylena Reed, Melanie M. Cobb, Gaia Skinbinski, Dorien A. Roosen et autres

Reproducibility of expression patterns in iPSC-derived cells from different labs is an important first step in ensuring replication of biochemical or functional assays that are performed in different labs. Here we show that reproducible gene expression patterns from iPSCs and iPSC-derived neurons …

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Generation of iPSC line from a Parkinson patient with PARK7 mutation and CRISPR-edited Gibco human episomal iPSC line to mimic PARK7 mutation

Melissa Conti Mazza, Alexandra G. Beilina, Dorien A. Roosen, David N. Hauser et autres

Mutations in the oncogene PARK7, which codes for DJ-1, have been associated with early-onset autosomal recessive Parkinson's disease (PD); however, the exact role of DJ-1 in PD remains elusive. Fibroblasts from a PD patient with a uniparental disomy, 1 bp deletion in …

us (code pays fourni par la source)

Sulfonated rhodamines as impermeable labelling substrates for cell surface protein visualization

Ramona Birke, Julia Ast, Dorien A. Roosen, Bettina Mathes et autres

ABSTRACT Sulfonated rhodamines that endow xanthene dyes with cellular impermeability are presented. We fuse charged sulfonates to red and far-red dyes to obtain Sulfo549 and Sulfo646, respectively, and further link these to SNAP- and Halo-tag substrates for protein self-labelling. Cellular impermeability is …

de, gb (code pays fourni par la source)

A Presynaptic Perspective on Transport and Assembly Mechanisms for Synapse Formation

Filiz Sila Rizalar, Dorien A. Roosen, Volker Haucke

de (code pays fourni par la source)

Extracellular clusterin limits the uptake of α‐synuclein fibrils by murine and human astrocytes

Alice Filippini, Veronica Mutti, Gaia Faustini, Francesca Longhena et autres

The progressive neuropathological damage seen in Parkinson's disease (PD) is thought to be related to the spreading of aggregated forms of α-synuclein. Clearance of extracellular α-synuclein released by degenerating neurons may be therefore a key mechanism to control the concentration of α-synuclein …

it, us (code pays fourni par la source)

The Parkinson’s Disease Protein LRRK2 Interacts with the GARP Complex to Promote Retrograde Transport to the trans-Golgi Network

Alexandra G. Beilina, Luis Bonet‐Ponce, Ravindran Kumaran, Jennifer J. Kordich et autres

Mutations in Leucine-rich repeat kinase 2 (LRRK2) cause Parkinson's disease (PD). However, the precise function of LRRK2 remains unclear. We report an interaction between LRRK2 and VPS52, a subunit of the Golgi-associated retrograde protein (GARP) complex that identifies a function of LRRK2 …

us, gb (code pays fourni par la source)

Comprehensive assessment of PINK1 variants in Parkinson's disease

Lynne Krohn, Francis P. Grenn, Mary B. Makarious, Jonggeol Jeffrey Kim et autres

ca, us (code pays fourni par la source)

Comprehensive assessment of PINK1 variants in Parkinson’s disease

Lynne Krohn, Francis P. Grenn, Mary B. Makarious, Jonggeol Jeffrey Kim et autres

Abstract Multiple genes have been associated with monogenic Parkinson’s disease and Parkinsonism syndromes. Mutations in PINK1 (PARK6) have been shown to result in autosomal recessive early onset Parkinson’s disease. In the past decade, several studies have suggested that carrying a single heterozygous …

ca, us (code pays fourni par la source)

Mutations in Auxilin cause parkinsonism via impaired clathrin-mediated trafficking at the Golgi apparatus and synapse

Dorien A. Roosen, Natalie Landeck, Luis Bonet‐Ponce, Jillian H. Kluss et autres

Abstract Parkinson’s disease (PD) is a common neurodegenerative motor disorder characterized in part by neuropathological lesions in the nigrostriatal pathway. Loss of function mutations in Auxilin, the major neuronal clathrin uncoating protein, cause an aggressive form of juvenile onset PD. How mutations …

us, gb (code pays fourni par la source)