Pontus Nordenfelt

NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations

Iván Hidalgo-Cenalmor, Adán Olguín-Olguín, Carolina Prieto, Johannes Kumra Ahnlide et autres

Integrating tissue-level organisation with sub-cellular resolution and molecular information often requires combining multiple microscopy modalities and scales. However, aligning images acquired with different modalities, settings, or instruments remains challenging. Here, we introduce NucleiSky, a microscopy image registration framework that utilises the spatial …

fi, pt, se (code pays fourni par la source)

Mechanical memory of confinement pressure governs expansion size in epithelial monolayers

Linn Engström, Simon K. Schnyder, Johannes Kumra Ahnlide, Valeriia Grudtsyna et autres

Abstract Epithelial tissues undergo rapid expansion during development, repair, and morphogenesis, yet how tissue-scale growth is coordinated to re-establish homeostasis remains unclear. Here, we show that large epithelial monolayers confined at a wide range of initial densities and mechanochemical states robustly converge …

se, jp, dk, nl (code pays fourni par la source)

NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations

Iván Hidalgo-Cenalmor, Adán Olguín-Olguín, Carolina Prieto, Johannes Kumra Ahnlide et autres

Integrating tissue-level organisation with sub-cellular resolution and molecular information often requires combining multiple microscopy modalities and scales. However, aligning images acquired with different modalities, settings, or instruments remains challenging. Here, we introduce NucleiSky, a microscopy image registration framework that utilises the spatial …

fi, pt, se (code pays fourni par la source)

Data associated with NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations

Iván Hidalgo-Cenalmor, Adán Olguín-Olguín, Carolina Prieto, Johannes Kumra Ahnlide et autres

This Zenodo archive contains raw microscopy images, segmentation masks, representative query images or crops, channel-transfer outputs, and benchmarking results associated with the manuscript: NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations Iván Hidalgo Cenalmor, Adán Olguín-Olguín, Carolina Prieto, Johannes …

pt, fi (code pays fourni par la source)

Data associated with NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations

Iván Hidalgo-Cenalmor, Adán Olguín-Olguín, Carolina Prieto, Johannes Kumra Ahnlide et autres

This Zenodo archive contains raw microscopy images, segmentation masks, representative query images or crops, channel-transfer outputs, and benchmarking results associated with the manuscript: NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations Iván Hidalgo Cenalmor, Adán Olguín-Olguín, Carolina Prieto, Johannes …

pt, fi (code pays fourni par la source)

ECM‐Stiffness Mediated Persistent Fibroblast Activation Requires Integrin and Formin Dependent Chromatin Remodeling

Swathi Packirisamy, Oscar André, Zhimeng Fan, Pontus Nordenfelt et autres

Transient activation of fibroblasts into contractile myofibroblasts is essential for extracellular matrix (ECM) production and remodeling during wound healing and tissue regeneration. While ECM-dependent mechanisms mediating transient activation is well studied, how fibroblasts switch from transient to a persistently activated state and …

se (code pays fourni par la source)

Smart microscopy: current implementations and a roadmap for interoperability

Lucien Hinderling, Hannah S. Heil, Alfredo Rates, Philipp Seidel et autres

Abstract Smart microscopy is transforming life sciences by automating experimental imaging workflows and enabling real-time adaptation based on feedback from images and other data streams. This shift increases throughput, improves reproducibility, and expands the functional capabilities of microscopes. However, the current landscape …

ch, se, nl, de, gb, fr, us (code pays fourni par la source)

Revisiting the streptococcal M protein: modern perspectives on antibody function, host interactions, and therapeutic targeting

Sebastian Wrighton, Pontus Nordenfelt

(GAS) causes extensive global morbidity and rising rates of invasive disease, for which clinical outcomes remain poor despite antibiotic treatment of susceptible strains. This limitation of current therapy underscores the need for alternative or adjunctive approaches. Antibody-based interventions represent a promising but …

se, hr, at (code pays fourni par la source)

From observation to understanding: A multi‐agent framework for smart microscopy

P. S. Kesavan, Pontus Nordenfelt

Smart microscopy represents a paradigm shift in biological imaging, moving from passive observation tools to active collaborators in scientific inquiry. Enabled by advances in automation, computational power, and artificial intelligence, these systems are now capable of adaptive decision-making and real-time experimental control. …

se (code pays fourni par la source)

Size-based sorting of dynamic bacterial clusters

Elham Akbari, Jason Paul Beech, Johannes Kumra Ahnlide, Sebastian Wrighton et autres

Group A Streptococcus (GAS) forms highly deformable aggregates with broad variations in size and morphology, complicating controlled separation and biological analysis. Reliable methods to isolate fractions of GAS clusters with defined properties are essential for studying host-pathogen interactions that depend on cluster …

se, at (code pays fourni par la source)

Integrin‐Piezo1 Axis Drives ECM Remodeling and Invasion of 3D Breast Epithelium (Adv. Sci. 5/2026)

Kabilan Sakthivel, Anna Kotowska, Zhimeng Fan, Ellen Juel Portner et autres

3D Mechanotransduction The cover image shows mammary acini progressing from polarized spheres to invasive, tumor-like structures as their surrounding matrix stiffens. Using an in-situ stiffening hydrogel system, Vinay S Swaminathan and co-workers demonstrate that ECM stiffening around normal 3D mammary acini activates …

Integrin‐Piezo1 Axis Drives ECM Remodeling and Invasion of 3D Breast Epithelium

Kabilan Sakthivel, Anna M. Kotowska, Zhimeng Fan, Ellen Juel Pørtner et autres

Stiffening of tissue is a hallmark of cancer progression, promoting invasive phenotypes through altered cell-extracellular matrix (ECM) interactions. However, how fully formed epithelial structures respond to mechanical cues within their native ECM environment remains poorly understood. Here, using a 3D in situ …

se, gb, dk (code pays fourni par la source)