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Determining orientation and direction of DNA sequences. A method by which fluorescence in situ hybridization can be made strand specific is described. Cell cultures are grown in a medium containing a halogenated nucleotide. The analog is partially incorporated in one DNA strand …
us (code pays fourni par la source)
Eman Mohammed Elbakrawy, Savneet Kaur Bains, Scott J. Bright, Raheem AL-Abedi et autres
Ionizing Radiation (IR), especially at high doses, induces cellular senescence in exposed cultures. IR also induces "bystander effects" through signals released from irradiated cells, and these effects include many of the same outcomes observed following direct exposure. Here, we investigate if radiation …
gb, Égypte, us (code pays fourni par la source)
Erin Robinson, Miles J. McKenna, Joel S. Bedford, Edwin H. Goodwin et autres
us (code pays fourni par la source)
Miles J. McKenna, Erin Robinson, Edwin H. Goodwin, Michael N. Cornforth et autres
us (code pays fourni par la source)
Ammar H. J. Al-Mayah, Scott J. Bright, Debbie A. Bowler, Predrag Slijepčević et autres
In directly irradiating cells, telomere metabolism is altered and similar effects have been observed in nontargeted cells. Exosomes and their cargo play dominant roles in communicating radiation-induced bystander effects with end points related to DNA damage. Here we report novel evidence that …
gb, us (code pays fourni par la source)
M. Lisa Phipps, Peter M. Goodwin, Jennifer S. Martinez, Edwin H. Goodwin
Chromosome ends are shielded from exonucleolytic attack and inappropriate end-joining by terminal structures called telomeres; these structures are potential targets for anticancer drugs. Telomeres are composed of a simple DNA sequence (5?-TTAGGG-3? in humans) repeated more than a thousand times, a short …
us (code pays fourni par la source)
Ammar H. J. Al-Mayah, Scott J. Bright, Kim L. Chapman, Sarah L. Irons et autres
gb, us (code pays fourni par la source)
F. Andrew Ray, Erin Robinson, Miles J. McKenna, Megumi Hada et autres
us (code pays fourni par la source)
F. Andrew Ray, Erin Zimmerman, Bruce W. S. Robinson, Michael N. Cornforth et autres
Chromosomal rearrangements are a source of structural variation within the genome that figure prominently in human disease, where the importance of translocations and deletions is well recognized. In principle, inversions-reversals in the orientation of DNA sequences within a chromosome-should have similar detrimental …
us (code pays fourni par la source)
Al-Mayah Ahj., M Kadhim, Mark A. Hill, Edwin H. Goodwin
Eli S. Williams, Michael N. Cornforth, Edwin H. Goodwin, Susan M. Bailey
Fluorescence in situ hybridization (FISH) has become a powerful tool for exploring genomes at the level of chromosomes. The procedure can be used to identify individual chromosomes, rearrangements between chromosomes, and the location within a chromosome of specific DNA sequences such as …
us (code pays fourni par la source)
Krastan B. Blagoev, Edwin H. Goodwin, Susan M. Bailey
Telomeres are a hotspot for sister chromatid exchange (T-SCE). Any biological consequence of this form of instability remained obscure until quantitative modeling revealed a link between elevated T-SCE rates and accelerated cellular replicative senescence. This work strongly suggests that progressive telomere erosion …
us (code pays fourni par la source)
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