Radiation-Induced Senescence Bystander Effect: The Role of Exosomes
Rattachement africain : gb, Égypte, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Ionizing Radiation (IR), especially at high doses, induces cellular senescence in exposed cultures. IR also induces "bystander effects" through signals released from irradiated cells, and these effects include many of the same outcomes observed following direct exposure. Here, we investigate if radiation can cause senescence through a bystander mechanism. Control cultures were exposed directly to 0, 0.1, 2, and 10 Gy. Unirradiated cells were treated with medium from irradiated cultures or with exosomes extracted from irradiated medium. The level of senescence was determined post-treatment (24 h, 15 days, 30 days, and 45 days) by β-galactosidase staining. Media from cultures exposed to all four doses, and exosomes from these cultures, induced significant senescence in recipient cultures. Senescence levels were initially low at the earliest timepoint, and peaked at 15 days, and then decreased with further passaging. These results demonstrate that senescence is inducible through a bystander mechanism. As with other bystander effects, bystander senescence was induced by a low radiation dose. However, unlike other bystander effects, cultures recovered from bystander senescence after repeated passaging. Bystander senescence may be a potentially significant effect of exposure to IR, and may have both beneficial and harmful effects in the context of radiotherapy.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Radiation-Induced Senescence Bystander Effect: The Role of Exosomes
- Date Crossref
- 27/07/2020
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Oxford Brookes University pays non établi dans la noticeUniversité ou école supérieure
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Egyptian Atomic Energy Authority Department of Radiation Physics Cairo, Égypte (code pays fourni par la source)Organisme public
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The University of Texas MD Anderson Cancer Center pays non établi dans la noticeÉtablissement de santé
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Angelina College pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Health and Life Sciences Department of Biological and Medical Sciences pays non établi dans la noticeUniversité ou école supérieure
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University of Texas MD Anderson Cancer Centre Department of Radiation Physics pays non établi dans la noticeUniversité ou école supérieure
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Angelina Biomedical Laboratories pays non établi dans la noticeInstitution
Oxford Brookes University, Department of Radiation Physics — Egyptian Atomic Energy Authority (Cairo, Égypte) et The University of Texas MD Anderson Cancer Center, avec 4 autres affiliations. Pays d’affiliation : Égypte.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.