Xinchen Chen

A genome-wide MAGIC kit for recombinase-independent mosaic analysis in Drosophila

Yifan Shen, Ann T Yeung, Payton Ditchfield, Elizabeth Korn et autres

Mosaic analysis has been instrumental in advancing developmental and cell biology. Most current mosaic techniques rely on exogenous site-specific recombination sequences that need to be introduced into the genome, limiting their application. Mosaic analysis by gRNA-induced crossing-over (MAGIC) was recently developed in …

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Author response: A genome-wide MAGIC kit for recombinase-independent mosaic analysis in Drosophila

Yifan Shen, Ann T Yeung, Payton Ditchfield, Elizabeth Korn et autres

A comprehensive toolkit enables genome-wide, recombinase-independent mosaic analysis in Drosophila, permitting clonal analysis of pericentromeric genes, deficiency chromosomes, and interspecific hybrids previously inaccessible to standard methods.

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A genome-wide MAGIC kit for recombinase-independent mosaic analysis in Drosophila

Yifan Shen, Ann T Yeung, Payton Ditchfield, Elizabeth Korn et autres

Mosaic analysis has been instrumental in advancing developmental and cell biology. Most current mosaic techniques rely on exogenous site-specific recombination sequences that need to be introduced into the genome, limiting their application. Mosaic analysis by gRNA-induced crossing-over (MAGIC) was recently developed in …

us (code pays fourni par la source)

A genome-wide MAGIC kit for recombinase-independent mosaic analysis in Drosophila

Yifan Shen, Ann T Yeung, Payton Ditchfield, Elizabeth Korn et autres

Abstract Mosaic analysis has been instrumental in advancing developmental and cell biology. Most current mosaic techniques rely on exogenous site-specific recombination sequences that need to be introduced into the genome, limiting their application. Mosaic analysis by gRNA-induced crossing-over (MAGIC) was recently developed …

us (code pays fourni par la source)

Author response: A genome-wide MAGIC kit for recombinase-independent mosaic analysis in Drosophila

Yifan Shen, Ann T Yeung, Payton Ditchfield, Elizabeth Korn et autres

Mosaic analysis has been instrumental in advancing developmental and cell biology. Most current mosaic techniques rely on exogenous site-specific recombination sequences that need to be introduced into the genome, limiting their application. Mosaic analysis by gRNA-induced crossing-over (MAGIC) was recently developed in …

A genome-wide MAGIC kit for recombinase-independent mosaic analysis in Drosophila

Ann T Yeung, Payton Ditchfield, Elizabeth Korn, R G H CLEMENTS et autres

Mosaic analysis has been instrumental in advancing developmental and cell biology. Most current mosaic techniques rely on exogenous site-specific recombination sequences that need to be introduced into the genome, limiting their application. Mosaic analysis by gRNA-induced crossing-over (MAGIC) was recently developed in …

us (code pays fourni par la source)

A genome-wide MAGIC kit for recombinase-independent mosaic analysis in Drosophila

Yifan Shen, Ann T Yeung, Payton Ditchfield, Elizabeth Korn et autres

ABSTRACT Mosaic analysis has been instrumental in advancing developmental and cell biology. Most current mosaic techniques rely on exogenous site-specific recombination sequences that need to be introduced into the genome, limiting their application. Mosaic analysis by gRNA-induced crossing-over (MAGIC) was recently developed …

us (code pays fourni par la source)

Phagocytosis-driven neurodegeneration through opposing roles of an ABC transporter in neurons and phagocytes

Xinchen Chen, Bei Wang, Ankita Sarkar, Nicolas Vergara Ruiz et autres

Lipid homeostasis is critical to neuronal survival. ATP-binding cassette A (ABCA) proteins are lipid transporters associated with neurodegenerative diseases. How ABCA transporters regulate lipid homeostasis in neurodegeneration is an outstanding question. Here we report that the Drosophila ABCA protein engulfment ABC transporter …

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Tissue-specific knockout in the Drosophila neuromuscular system reveals ESCRT’s role in formation of synapse-derived extracellular vesicles

Xinchen Chen, Sarah L. Perry, Bei Wang, Elizabeth Loxterkamp et autres

Tissue-specific gene knockout by CRISPR/Cas9 is a powerful approach for characterizing gene functions during development. However, this approach has not been successfully applied to most Drosophila tissues, including the Drosophila neuromuscular junction (NMJ). To expand tissue-specific CRISPR to this powerful model system, …

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Phagocytosis-driven neurodegeneration through opposing roles of an ABC transporter in neurons and phagocytes

Xinchen Chen, Bei Wang, Ankita Sarkar, Zixian Huang et autres

SUMMARY Lipid homeostasis is critical to the survival of neurons. Lipid transporters from the ATP-binding cassette A (ABCA) subfamily are important regulators of lipid trafficking and are associated with multiple neurodegenerative diseases. How ABCA transporters regulate specific aspects of lipid homeostasis to …

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Tissue-specific knockout in Drosophila neuromuscular system reveals ESCRT’s role in formation of synapse-derived extracellular vesicles

Xinchen Chen, Sarah L. Perry, Bei Wang, Shuran Wang et autres

ABSTRACT Tissue-specific gene knockout by CRISPR/Cas9 is a powerful approach for characterizing gene functions in animal development. However, this approach has been successfully applied in only a small number of Drosophila tissues. The Drosophila motor nervous system is an excellent model system …

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The Drosophila chemokine–like Orion bridges phosphatidylserine and Draper in phagocytosis of neurons

Hui Ji, Bei Wang, Yifan Shen, David Labib et autres

Phagocytic clearance of degenerating neurons is triggered by “eat-me” signals exposed on the neuronal surface. The conserved neuronal eat-me signal phosphatidylserine (PS) and the engulfment receptor Draper (Drpr) mediate phagocytosis of degenerating neurons in Drosophila . However, how PS is recognized by …

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