Punam Bisht

A human blood-brain barrier model reveals pericytes as critical regulators of viral neuroinvasion

Alexsia Richards, Andrew Khalil, Punam Bisht, Troy W. Whitfield et autres

cellular interactions at the BBB. We used this model to evaluate the pathological consequences of BBB exposure to highly neuroinvasive flaviviruses. Our results identify a previously undescribed role for NCC-PCs in maintaining BMEC barrier integrity during infection and reducing the spread of …

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A novel human blood-brain barrier model reveals pericytes as critical regulators of viral neuroinvasion

Alexsia Richards, Andrew Khalil, Punam Bisht, Troy W. Whitfield et autres

Abstract The blood-brain barrier (BBB) plays a vital role in regulating the passage of biomolecules between the bloodstream and the central nervous system (CNS) while also protecting the CNS from pathogens. Pericytes reside at the interface between the endothelial cells that form …

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Multi-species genome-wide CRISPR screens identify conserved suppressors of cold-induced cell death

Breanna Lam, Kathrin M Kajderowicz, Heather R. Keys, Julian Roessler et autres

Summary Cells must adapt to environmental changes to maintain homeostasis. One of the most striking environmental adaptations is entry into hibernation during which core body temperature can decrease from 37°C to as low at 4°C. How mammalian cells, which evolved to optimally …

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Multi-species genome-wide CRISPR screens identify conserved suppressors of cold-induced cell death

Breanna Lam, Kathrin M Kajderowicz, Heather R. Keys, Julian Roessler et autres

Summary Cells must adapt to environmental changes to maintain homeostasis. One of the most striking environmental adaptations is entry into hibernation during which core body temperature can decrease from 37°C to as low at 4°C. How mammalian cells, which evolved to optimally …

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Author response: Multi-species genome-wide CRISPR screens identify conserved suppressors of cold-induced cell death

Breanna Lam, Kathrin M Kajderowicz, Heather R. Keys, Julian Roessler et autres

Cells must adapt to environmental changes to maintain homeostasis. One of the most striking environmental adaptations is entry into hibernation during which core body temperature can decrease from 37°C to as low at 4°C. How mammalian cells, which evolved to optimally function …

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Abortive infection of bat fibroblasts with SARS-CoV-2

Punam Bisht, Michael D. Gallagher, M. Inmaculada Barrasa, Julie Boucau et autres

Bats are tolerant to highly pathogenic viruses such as Marburg, Ebola, and Nipah, suggesting the presence of a unique immune tolerance toward viral infection. Here, we compared severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection of human and bat ( Rhinolophus ferrumequinum …

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Multi-species genome-wide CRISPR screens identify conserved suppressors of cold-induced cell death

Breanna Lam, Kathrin M Kajderowicz, Heather R. Keys, Julian Roessler et autres

Cells must adapt to environmental changes to maintain homeostasis. One of the most striking environmental adaptations is entry into hibernation during which core body temperature can decrease from 37°C to as low at 4°C. How mammalian cells, which evolved to optimally function …

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Commercial human 3D corneal epithelial equivalents for modeling epithelial infection in herpes keratitis

Tatiana Borodianskiy-Shteinberg, Punam Bisht, Biswajit Das, Paul R. Kinchington et autres

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Genomic and biological variation in bat IFNs: An antiviral treatment approach

Laith N. AL‐Eitan, Ahmad Mihyar, Liguo Zhang, Punam Bisht et autres

Bat-borne viruses have attracted considerable research, especially in relation to the Covid-19 pandemic. Although bats can carry multiple zoonotic viruses that are lethal to many mammalian species, they appear to be asymptomatic to viral infection despite the high viral loads contained in …

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Human iPS cell-derived sensory neurons can be infected by SARS-CoV-2

Anthony Flamier, Punam Bisht, Alexsia Richards, Danielle L. Tomasello et autres

COVID-19 has impacted billions of people since 2019 and unfolded a major healthcare crisis. With an increasing number of deaths and the emergence of more transmissible variants, it is crucial to better understand the biology of the disease-causing virus, the SARS-CoV-2. Peripheral …

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LINE1-Mediated Reverse Transcription and Genomic Integration of SARS-CoV-2 mRNA Detected in Virus-Infected but Not in Viral mRNA-Transfected Cells

Liguo Zhang, Punam Bisht, Anthony Flamier, M. Inmaculada Barrasa et autres

SARS-CoV-2 sequences can be reverse-transcribed and integrated into the genomes of virus-infected cells by a LINE1-mediated retrotransposition mechanism. Whole-genome sequencing (WGS) methods detected retrotransposed SARS-CoV-2 subgenomic sequences in virus-infected cells overexpressing LINE1, while an enrichment method (TagMap) identified retrotranspositions in cells that …

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LINE1-mediated reverse transcription and genomic integration of SARS-CoV-2 mRNA detected in virus-infected but not in viral mRNA-transfected cells

Liguo Zhang, Punam Bisht, Anthony Flamier, M. Inmaculada Barrasa et autres

SARS-CoV-2 sequences can be reverse-transcribed and integrated into the genomes of virus-infected cells by a LINE1-mediated retrotransposition mechanism. Whole genome sequencing (WGS) methods detected retrotransposed SARS-CoV-2 subgenomic sequences in virus-infected cells overexpressing LINE1, while an enrichment method (TagMap) identified retrotranspositions in cells …

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