Matthias Steglich

Post-Weaning Gut Microbiota Colonization Reveals Divergent Recovery of Skeletal Muscle and Peripheral Nerves

Sonia Calabrò, Davide Pellegrino, Chiara Cicconetti, Svenja Kankowski et autres

Abstract We previously demonstrated that the absence of a complex gut microbiota (CGM) impairs the postnatal development of peripheral nerves and motor targets in germ-free (GF) mice. In this study, we investigated whether establishing a complex gut microbiota after weaning could reverse …

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High-resolution taxonomic profiling and metatranscriptomics identify microbial, biochemical, host, and ecological factors in peri-implant disease

Szymon Piotr Szafrański, Amruta Joshi, Matthias Steglich, Ines Yang et autres

ABSTRACT Biofilm-associated diseases like peri-implant mucositis (PIM) and peri-implantitis (PI) are significant clinical challenges affecting millions of dental implant patients globally. Although studies have described the role of microbial, host, or environmental factors in disease development, their complex interplay, particularly during dysbiosis, …

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High-resolution taxonomic profiling and metatranscriptomics identify microbial, biochemical, host, and ecological factors in peri-implant disease

Szymon P. Szafranski, Amruta A. Joshi, Matthias Steglich, Ines Yang et autres

ABSTRACT Biofilm-associated diseases like peri-implant mucositis (PIM) and peri-implantitis (PI) are significant clinical challenges affecting millions of dental implant patients globally. Although studies have described the role of microbial, host, or environmental factors in disease development, their complex interplay, particularly during dysbiosis, …

Effects of elexacaftor/tezacaftor/ivacaftor on the nasal microbial metagenome in cystic fibrosis

Tom L. Hertramph, Marie Dorda, Sophia Theres Pallenberg, Annette Sauer‐Heilborn et autres

ABSTRACT Mutation-specific cystic fibrosis (CF) transmembrane conductance regulator (CFTR) modulator therapy with elexacaftor/tezacaftor/ivacaftor (ETI) has dramatically improved clinical outcomes for people with CF (pwCF), yet its impact on the nasal microbial metagenome remains insufficiently understood. This prospective, post-approval study investigated the impact …

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Human ILC methylomes uncover stable lineage codes and disease-linked ILC2 regulators in allergy

Ayush Jain, Elia C. Diem, Chia-wen Lu, Matthias Steglich et autres

Abstract The transcriptional programs of human ILCs are increasingly defined, but the DNA-methylation landscapes that stabilize their identity and function remain poorly understood. Here, we generated genome-wide DNA methylomes of human NK cells, ILC1, ILC2, and ILC3 from blood and lymphoid tissues. …

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Commensal microflora coating endows implants with biofilm-repellent, immunomodulatory and osteogenic properties

Muhammad Imran Rahim, Shehneela Baseer, Daniela Paasch, Matthias Steglich et autres

Bacteria often form biofilms on biomaterials, and these biomaterial-associated infections then become highly resistant to antibiotics and the host immune system. Biofilm-driven implant failures underscore the urgent need for surface modifications that can concurrently prevent microbial colonization, modulate immune responses, and stimulate …

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Phylogeny‐Aware Metabologenomics Accurately Assigns Natural Products to Biosynthetic Gene Clusters

Judith Boldt, Christoph Porten, F. P. Jake Haeckl, Joachim J. Hug et autres

ABSTRACT Tens of thousands of biosynthetic gene clusters (BGCs) have been identified in microbial genomes, but the vast majority of associated natural products (NPs) and their underlying biosyntheses remain unknown. Metabologenomics approaches integrate genomic and metabolomic datasets to statistically associate BGCs to …

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Integrative microbiome- and metatranscriptome-based analyses reveal diagnostic biomarkers for peri-implantitis

Amruta Joshi, Szymon Piotr Szafrański, Matthias Steglich, Ines Yang et autres

Peri-implantitis is a severe biofilm-associated infection affecting millions worldwide. This cross-sectional study aimed to identify taxonomic and functional biomarkers that reliably indicate peri-implantitis by utilizing paired data from full length 16S rRNA gene amplicon sequencing (full-16S) and metatranscriptomics (RNAseq) in 48 biofilm …

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The Submucosal Microbiome Correlates with Peri-implantitis Severity

Aaroh Anand Joshi, Szymon Piotr Szafrański, Matthias Steglich, Ines Yang et autres

Peri-implantitis is an increasingly prevalent chronic inflammatory disease of the peri-implant tissue. A key etiologic factor for peri-implantitis is the submucosal biofilm, which may further drive clinical severity and accelerate disease progression. The present cross-sectional study aimed to characterize the compositional (full-length …

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Invasive species drive polymicrobial resistance to amoxicillin in oral biofilms through β-lactamase release

Amy L. Seidel, Matthias Steglich, Taoran Qu, Amruta Joshi et autres

Abstract Since bacterial biofilms often cause refractory infections, antimicrobial susceptibility testing (AST) is highly desirable even for oral peri-implant biofilms. However, characterization of polymicrobial drug resistance is challenging due to high diversity and complexity of these biofilms. In this work, we developed …

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Integrative microbiome- and metatranscriptome-based analyses reveal diagnostic biomarkers for peri-implantitis

Amruta Joshi, Szymon Piotr Szafrański, Matthias Steglich, Ines Yang et autres

Abstract Peri-implantitis is a severe biofilm-associated infection of the tissues around dental implants that increases the risk of implant failure. The prognosis of peri-implantitis treatment is compromised by the resistance of well-organized and mature bacterial biofilms. Thus, early diagnosis of a pathogenic …

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EPS8.03Effects of elexacaftor/tezacaftor/ivacaftor on the nasal microbial metagenome in cystic fibrosis

T.L. Hertramph, Silvia Genovese, Marie Dorda, Sophia Theres Pallenberg et autres

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