D Tabakov

The DR-DQ HLA haplotypes dominant-recessive interplay leads to the heterogeneity of antibody immune response in T1D

Elizaveta A. Telnaya, Ekaterina S. Petriaikina, Zoia G. Antysheva, Dmitry I. Tychinin et autres

Abstract Type 1 diabetes mellitus (T1D) is an autoimmune disorder defined by the presence of islet-specific autoantibodies (AAb). Growing evidence indicates substantial heterogeneity in T1D pathogenesis, reflected in divergent AAb profiles across patient subgroups. We integrated ELISA-based measurements of four major islet …

Identifying a Common Autoimmune Gene Core as a Tool for Verifying Biological Significance and Applicability of Polygenic Risk Scores

Victoria Sergeevna Shchekina, Nikita Aleksandrovich Batashkov, Anna Arkadievna Maznina, Julia Aleksandrovna Krupinova et autres

Polygenic autoimmune diseases (ADs) have several common features that are caused by a complex interplay of genetic and environmental factors. Common pathophysiological mechanisms include dysregulation of the immune system, chronic inflammation, and epigenetic changes influenced by external factors. For the prediction of …

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Leveraging two novel droplet−based single−cell RNA Sequencing platforms: a comparative study with 10x genomics

Alesia Maluchenko, Ekaterina Avsievich, Ia. S. Zvorygina, Zoya Antysheva et autres

BACKGROUND: Currently, single cell transcriptome profiling technology is gaining popularity, with an increasing number of novel platforms emerging. The development of new technologies is aimed at improving data quality, scale, cost, or labor intensity. Given the rapid evolution of these technologies, a …

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Preclinical Diagnosis of Type 1 Diabetes: Reality or Utopia

Tatiana Alekseevna Marakhovskaya, D Tabakov, О. В. Глушкова, Zoya Antysheva et autres

-cells, predominantly manifesting in childhood or adolescence. The lack of clearly interpretable biological markers in the early stages, combined with the insidious onset of the disease, poses significant challenges to early diagnosis and the implementation of preventive strategies. The applicability of classic …

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Single-cell RNA sequencing in studies of type 1 diabetes mellitus: modern state-of-the-art and technical peculiarities

Azat Abdullatypov, О. В. Глушкова, Ekaterina S. Petriaikina, Viktor P. Bogdanov et autres

-cells and loss of insulin production ability. Pathogenesis of T1DM is a complex process involving different types of immune cells, particularly, T-lymphocytes (including effector cells, Thelpers, regulatory lymphocytes, MAIT cells), B-lymphocytes, natural killers, monocytes, dendritic cells, and other minor cellular populations that …

ru, cz (code pays fourni par la source)

Diagnosis of cancer, autoimmune and infectious diseases and prediction of the therapy effectiveness based on the individual’s immunotype

D Tabakov, Anna A. Maznina, Ekaterina A. Astakhova, Е. Н. Захарова et autres

Immune system plays a central role in the pathogenesis of cancer and autoimmune diseases. An entire field has emerged to identify separate minor cell subpopulations carrying potential molecular targets or activation markers to study their prognostic role in disease progression and severity …

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Spectral Flow Cytometry: The Current State and Future of the Technology

Elena Astakhova, Anna Gubaeva, Daria A. Naumova, Ann Egorova et autres

Flow cytometry is a powerful and widely used tool for the analysis of various cell populations, but its capabilities are severely limited by the need to apply correction of fluorescent signals from near or similar fluorochromes when analyzing multicolor panels. Spectral flow …

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Clinical trial links oncolytic immunoactivation to survival in glioblastoma

Alexander Ling, Isaac H. Solomon, Ana Montalvo Landivar, Hiroshi Nakashima et autres

Abstract Immunotherapy failures can result from the highly suppressive tumour microenvironment that characterizes aggressive forms of cancer such as recurrent glioblastoma (rGBM) 1,2 . Here we report the results of a first-in-human phase I trial in 41 patients with rGBM who were …

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1215P Comparative analysis of cfDNA liquid biopsy and tumor-based next-generation sequencing (NGS) approaches

A. V. Yudina, Sergey Starikov, А А Ефремов, Danielle Sookiasian et autres

Spatial mapping of human hematopoiesis at single-cell resolution reveals aging-associated topographic remodeling

Aleksandr Sarachakov, Arina Varlamova, Viktor Svekolkin, Margarita Polyakova et autres

ABSTRACT: The spatial anatomy of hematopoiesis in the bone marrow (BM) has been extensively studied in mice and other preclinical models, but technical challenges have precluded a commensurate exploration in humans. Institutional pathology archives contain thousands of paraffinized BM core biopsy tissue …

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Longitudinal Molecular Analysis of Tumor Exome and Transcriptome to Evaluate Clonal Evolution and Identify Novel Therapeutic Targets in Thymoma

Christopher S. Nabel, Yin P. Hung, Anna Kurilovich, Aleksandra Lopareva et autres

The rarity of thymic epithelial tumors and limited preclinical modeling constrain our understanding of disease biology and the advancement of rational treatment strategies.Tumor mutational profiling has demonstrated sporadic activating mutations in targetable tyrosine kinases that have not been successfully targeted outside of …

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Single Cell Resolution Spatial Mapping of Human Hematopoiesis Reveals Aging-Associated Topographic Remodeling

Aleksandr Sarachakov, Arina Varlamova, Viktor Svekolkin, Ilia Galkin et autres

ABSTRACT The spatial anatomy of hematopoiesis in bone marrow has been extensively studied in mice and other preclinical models, but technical challenges have precluded a commensurate exploration in humans. Institutional pathology archives contain thousands of paraffinized bone marrow core biopsy tissue specimens, …

us (code pays fourni par la source)