2026
preprint
OpenAlex
L. Schüler, Robert Winkler, José Gonçalves-Dias, K Schuschel et autres
Abstract Pediatric acute myeloid leukemia (AML) is driven by aberrant transcriptional programs sustained by poorly defined cis-regulatory mechanisms. To systematically identify functional enhancer dependencies, we developed an integrative enhancer discovery strategy that combines H3K27ac CUT&Tag profiling, enhancer-associated transcription, and CRISPR interference (CRISPRi) …
de
(code pays fourni par la source)
Accès ouvert
2026
preprint
OpenAlex
Marius Külp, Mireia Osuna Lopez, Anna-Sophia Wiegand, Sebastian P. Perner et autres
Abstract How transfer RNA (tRNA) expression changes as hematopoietic stem cells are specified into human blood cell types remains unknown. tRNAs translate the 64-codon genetic code into the 21-amino acid protein code, and their relative abundance can influence proteomic output. Here, we …
de
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Mauro Siragusa, Johannes Graumann, Carsten Kuenne, Stefan Günther et autres
AIMS: Microproteins (miPs) translated from small open reading frames (smORFs) are crucial regulators of cell function. However, the expression and function of miPs in endothelial cells and alterations in miP expression linked with inflammation and cardiovascular disease, remain largely unexplored. METHODS AND …
de, gb, se
(code pays fourni par la source)
Accès ouvert
2026
preprint
OpenAlex
Dewi Moonen, Annique Claringbould, Andreas R. Gschwind, Stefan Schrod et autres
Abstract CD4+ T cells harbor a disproportionate enrichment of immune disease risk loci and represent the primary cellular context for immune disease biology, yet the genes and regulatory programs these variants affect remain largely unknown. We combined targeted Perturb-seq of 1,032 cis …
gb, de, nl, us, ch
(code pays fourni par la source)
Accès ouvert
2026
dataset
OpenAlex
Dewi P. I. Moonen, Annique Claringbould, Andreas Gschwind, Stefan Schrod et autres
Single cell RNA-seq from naive CD4+ T cells isolated from blood from three healthy female donors, stimulated with anti-CD3/CD28 beads, infected with lentiviral dCas9-ZIM3-KRAB and a gRNA library and then reactivated. CRE screen: 1,032 putative CREs are perturbed with CRISPRi and the …
nl, us, gb, de, ch
(code pays fourni par la source)
Accès ouvert
2026
dataset
OpenAlex
Dewi P. I. Moonen, Annique Claringbould, Andreas Gschwind, Stefan Schrod et autres
Single cell RNA-seq from naive CD4+ T cells isolated from blood from three healthy female donors, stimulated with anti-CD3/CD28 beads, infected with lentiviral dCas9-ZIM3-KRAB and a gRNA library and then reactivated. CRE screen: 1,032 putative CREs are perturbed with CRISPRi and the …
nl, us, gb, de, ch
(code pays fourni par la source)
Accès ouvert
2026
erratum
OpenAlex
Arghya Dutta, Alberto Cristiani, Siddhanta Nikte, Jonathan Eisert et autres
In the version of the article initially published, Ronay Cetin (Institute of Biochemistry II, Faculty of Medicine, Goethe University, Theodor-Stern-Kai 7, Frankfurt am Main, Germany) did not appear in the author list and is now included in the HTML and PDF versions …
de, in
(code pays fourni par la source)
2026
conference-paper
OpenAlex
A Krumme, Yves Matthess, Thorben Huth, E Eiteneuer et autres
Hepatocellular carcinoma (HCC), the predominant form of liver cancer, is a leading cause of cancer-related mortality worldwide. Inflammation is central to HCC progression, with the interleukin-6 (IL-6)/STAT3 signaling pathway being frequently hyperactivated. Loss of heterozygosity (LoH) on chromosome 8p (chr8p) is a …
de
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Arghya Dutta, Alberto Cristiani, Siddhanta Nikte, Jonathan Eisert et autres
E3 ubiquitin ligases are vital enzymes that define the ubiquitin code in cells. Beyond promoting protein degradation to maintain cellular health, they also mediate non-degradative processes like DNA repair, signaling, and immunity. Despite their therapeutic potential, a comprehensive framework for understanding the …
de, in
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Maria-Kyriaki Drekolia, Yunyun Chen, Yali Du, Janina Wittig et autres
Abstract Whether, when, and how lymphatic vessels undergo cell death remains poorly understood. Here we identify ferroptosis as a physiological, cell-intrinsic regulator of the lymphatic endothelial cell survival during development and following injury, in stark contrast to the resilient organotypic blood endothelial …
de, ye, cn, kr, gb, il
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Accès ouvert
2025
preprint
OpenAlex
Alec Geßner, Adrien Jolly, Tjeerd Sijmonsma, Yves Matthess et autres
Abstract While a differentiation hierarchy with leukemia-initiating stem cells (LICs) at the apex is well documented for acute myeloid leukemia, the existence of LICs and their trajectories in B cell acute lymphoblastic leukemia (B-ALL) are debated. B-ALL is a malignant disease displaying …
de
(code pays fourni par la source)
2025
conference-paper
OpenAlex
Linda A. Schuler, Robert Winkler, J Gonçalves-Dias, K Schuschel et autres
Although survival rates in pediatric AML (pAML) increased, a deeper understanding of epigenetic oncogene regulation through long non-coding RNAs opens avenues for novel treatments. In our study, we explore the AML transcriptome landscape by comparing RNA sequencing of pediatric AML patients to …
de
(code pays fourni par la source)