Juan Huang

Local genetic distance-guided qPCR method for genotyping canine/feline coronaviruses

Deming Liu, Wenwen Yang, Yi Xing Liu, Shiyu Zeng et autres

Canine coronavirus (CCoV) and feline coronavirus (FCoV) are highly infectious in companion animals. Nevertheless, due to genomic recombination and mutagenesis of the two CoVs, these difficulties dampen their precise diagnosis especially by genotypes. Herein, we addressed these problems and developed a simple …

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Artificial intelligence–assisted quantification of fundus tessellation in early-onset high myopia

Yiming Guo, Kexin Wu, Juan Huang, Bo Ma et autres

Purpose To quantitatively evaluate fundus tessellation density (FTD) in children with early-onset high myopia (eo-HM) using artificial intelligence (AI)–assisted image analysis and to explore its association with axial length (AL). Methods This cross-sectional study included children aged ≤6 years with eo-HM, defined …

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A targeted mutation in ICP8 attenuates duck plague virus by disrupting replication and proposes a vaccine strategy

Ying Juan Wu, Anyang Sun, Haichuan Zhan, Zhiyuan Liang et autres

Controlling duck plague (DP) is essential for waterfowl health and industry productivity. The herpesviral single-stranded DNA-binding protein ICP8 is essential for replication compartment assembly, yet its exploitation for vaccine development in avian herpesviruses is limited. In this study, we generated two ICP8 …

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NS2B is indispensable for NS3/NS5 interaction of Tembusu virus in living cells

Yu He, Yi Shi, Zhen Wu, Tao Wang et autres

Tembusu virus (TMUV) causes egg-drop syndrome and neurological signs in duck, goose and chicken, posing a great threat to the waterfowl industry. In the present study, we established an in vivo protein-protein interaction system for the NS2B-3 and NS5, together with ​co-immunoprecipitation …

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Perceived infertility-related stress, partner attachment, and psychological distress among women undergoing in-vitro fertilization treatment: a latent profile and mediation analysis

Dan Liu, Shunding Hu, Bing Fu, Qinjing Yang et autres

BACKGROUND: The heterogeneity of infertility-related stress and its psychological impact among women undergoing in vitro fertilization (IVF) treatment remain insufficiently understood. This study aims to identify distinct stress profiles and examine the mediating role of partner attachment in the link between stress …

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Identification of a novel linezolid resistance gene, cfr(F), in Riemerella anatipestifer

Yihua Zhang, Zhishuang Yang, Mingshu Wang, Renyong Jia et autres

• A novel linezolid resistance gene, cfr (F), was identified in R. anatipestifer . • cfr (F) confers cross-resistance to PhLOPS A antibiotics, including linezolid. • R. anatipestifer is a potential reservoir for linezolid resistance gene dissemination.

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DPV UL46 activates EGFR-PI3K-AKT pathway to antagonize innate immunity by interfering with cGAS-STING-TBK-IRF7 Signalsome formation

Yanming Tian, Bin Tian, Dongjie Cai, Zhen Xiao et autres

Duck plague virus (DPV), a representative species of the Alphaherpesvirinae subfamily, poses significant economic and biosecurity threats to poultry production. Although the EGFR-PI3K-AKT signaling pathway is known to regulate diverse cellular processes-including cell growth, protein synthesis and immune regulation-and plays a significant …

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Establishment an echovirus 6 infection model based on hFcRn transgenic mice

Fei Li, Yiliang Fu, Luci Huang, Hanwen Zhang et autres

Echovirus 6 (E6) infection, a member of enterovirus, can cause severe neurological complications, particularly viral meningitis and encephalitis in children. However, the shortage of effective animal models has substantially impeded research on its pathogenesis and the advancement of therapeutic strategies. This study …

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Orchestration of hepatocyte inflammatory responses by monocytes during acute viral hepatitis in ducks in vitro

Xiaoming Lin, Yajia Gou, Yi Liu, Jingwen Dong et autres

Severe liver inflammation, in which the interaction between circulating immune cells and hepatocytes plays a crucial role, is a major concern in acute viral hepatitis. Nevertheless, the mechanisms by which these circulating immune cells drive hepatocyte inflammatory responses remain inadequately understood. In …

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High TSH levels during TSH suppression therapy in DTC postoperative patients are associated with low DIO2 expression in the thyroid and impaired thyroid hormone sensitivity

Jialu Wu, Juan Huang, Zhe Yan, Anqi Yuan et autres

Introduction: Some patients with differentiated thyroid cancer (DTC) exhibit persistently elevated TSH levels despite undergoing TSH suppression therapy after total thyroidectomy. This study aims to investigate the expression of type 2 deiodinase (DIO2) in thyroid tissues, as well as central and peripheral …

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Low-molecular-weight heparin alleviates L-arginine-induced acute pancreatitis by modulating lipid metabolism and inflammatory responses in a rat model

Jianmei Zeng, Kejin Li, Limao Xu, Chengfeng Wu et autres

OBJECTIVE: This study investigates the therapeutic potential of low-molecular-weight heparin (LMWH) in acute pancreatitis (AP), focusing on its effects on lipid metabolism, inflammatory responses, and pancreatic tissue integrity. The study also explores the involvement of the PI3K/Akt pathway in mediating LMWH’s protective …

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DPV UL38 stabilizes MFN2 to subvert MAVS-mediated antiviral immunity in ducks

Bin Tian, Xuetong Wang, Shuyi He, Dongjie Cai et autres

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