Local genetic distance-guided qPCR method for genotyping canine/feline coronaviruses
Rattachement africain : cn. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Canine coronavirus (CCoV) and feline coronavirus (FCoV) are highly infectious in companion animals. Nevertheless, due to genomic recombination and mutagenesis of the two CoVs, these difficulties dampen their precise diagnosis especially by genotypes. Herein, we addressed these problems and developed a simple real-time quantitative polymerase chain reaction (qPCR) method capable of genotyping major CCoV and FCoV, and partially surveyed the current epidemiological prevalence in dog and cat populations. Exploiting both evolutionary analysis and genome-wide genetic distance analysis, we identified locally conserved genomic regions that are specific to each genotype, including representative CCoV (sub)genotypes (I, IIa, IIb and IIc) and FCoV genotypes (I and II). By targeting these conserved regions, genotype-specific primer pairs were designed and cross-validated by in silico BLAST analysis, PCR melt curve and sequencing analysis. These primer pairs were integrated into the development of a simple qPCR approach and applied for genotyping potential CCoVs and FCoVs in Chengdu, China. We found 62.71% positivity in symptomatic dogs ( n = 59) and 66.00% positivity in symptomatic cats ( n = 50), with CCoV-I and FCoV-I being major genotypes in their native hosts during 2024–2026. Interestingly, many dogs and cats respectively tested positive for multiple CCoV and FCoV genotypes, with cross-host detection of FCoV RNA in dogs and CCoV RNA in cats. Collectively, we established a genotyping qPCR approach that enables the detection of CCoV and FCoV (sub)genotypes within a single analytical workflow, using one reaction per genotype.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Local genetic distance-guided qPCR method for genotyping canine/feline coronaviruses
- Date Crossref
- 04/08/2026
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Sichuan Agricultural University Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province pays non établi dans la noticeUniversité ou école supérieure
-
State Administration for Market Regulation pays non établi dans la noticeOrganisme public
-
Ministry of Education of the People's Republic of China pays non établi dans la noticeOrganisme public
-
College of Veterinary Medicine Institute of Veterinary Medicine and Immunology pays non établi dans la noticeUniversité ou école supérieure
-
Chengdu Institute of Food Inspection pays non établi dans la noticeStructure de recherche
-
Key Laboratory of Monitoring and Assessment on Novel Food Raw Materials pays non établi dans la noticeStructure de recherche
-
Key Laboratory of Agricultural Bioinformatics pays non établi dans la noticeStructure de recherche
Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province — Sichuan Agricultural University, State Administration for Market Regulation et Ministry of Education of the People's Republic of China, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.