Gut microbiota contributes to protection against porcine deltacoronavirus infection in piglets by modulating intestinal barrier and microbiome
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BACKGROUND: Gut microbiota plays a critical role in counteracting enteric viral infection. Our previous study demonstrated that infection of porcine deltacoronavirus (PDCoV) disturbs gut microbiota and causes intestinal damage and inflammation in piglets. However, the influence of gut microbiota on PDCoV infection remains unclear. RESULTS: Firstly, the relationship between gut microbiota and disease severity of PDCoV infection was evaluated using 8-day-old and 90-day-old pigs. The composition of gut microbiota was significantly altered in 8-day-old piglets after PDCoV infection, leading to severe diarrhea and intestinal damage. In contrast, PDCoV infection barely affected the 90-day-old pigs. Moreover, the diversity (richness and evenness) of microbiota in 90-day-old pigs was much higher compared to the 8-day-old piglets, suggesting the gut microbiota is possibly associated with the severity of PDCoV infection. Subsequently, transplanting the fecal microbiota from the 90-day-old pigs to the 3-day-old piglets alleviated clinical signs of PDCoV infection, modulated the diversity and composition of gut microbiota, and maintained the physical and chemical barrier of intestines. Additionally, metabolomic analysis revealed that the fecal microbiota transplantation (FMT) treatment upregulated the swine intestinal arginine biosynthesis, FMT significantly inhibited the inflammatory response in piglet intestine by modulating the TLR4/MyD88/NF-κB signaling pathway. CONCLUSIONS: PDCoV infection altered the structure and composition of the gut microbiota in neonatal pigs. FMT treatment mitigated the clinical signs of PDCoV infection in the piglets by modulating the gut microbiota composition and intestinal barrier, downregulating the inflammatory response. The preventive effect of FMT provides novel targets for the development of therapeutics against enteropathogenic coronaviruses. Video Abstract.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Gut microbiota contributes to protection against porcine deltacoronavirus infection in piglets by modulating intestinal barrier and microbiome
- Date Crossref
- 07/04/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Henan Agricultural University pays non établi dans la noticeUniversité ou école supérieure
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Yanan University Affiliated Hospital pays non établi dans la noticeÉtablissement de santé
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Ministry of Education of the People's Republic of China pays non établi dans la noticeOrganisme public
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Zhengzhou People's Hospital pays non établi dans la noticeÉtablissement de santé
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College of Veterinary Medicine pays non établi dans la noticeUniversité ou école supérieure
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College of Sport pays non établi dans la noticeUniversité ou école supérieure
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Henan Province Key Laboratory for Animal Food Pathogens Surveillance pays non établi dans la noticeStructure de recherche
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Ministry of Education Key Laboratory for Animal Pathogens and Biosafety pays non établi dans la noticeStructure de recherche
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Longhu Laboratory of Henan Province pays non établi dans la noticeStructure de recherche
Henan Agricultural University, Yanan University Affiliated Hospital et Ministry of Education of the People's Republic of China, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.