Exercise induces dynamic changes in intra-articular metabolism and inflammation associated with remodeling of the infrapatellar fat pad in mice
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Le résumé fourni par la source
We hypothesized that daily exercise promotes joint health by upregulating anti-inflammatory mediators via adaptive molecular and metabolic changes in the infrapatellar fat pad (IFP). We tested this hypothesis by conducting time-resolved analyses between 1 and 14 days of voluntary wheel running exercise in C57BL/6J mice. IFP structure and cellularity were evaluated by histomorphology, picrosirius red collagen staining, and flow cytometry analysis of stromal vascular fraction cells. Joint inflammation and metabolism were evaluated by multiplex gene expression analysis of synovium-IFP tissue and synovial fluid metabolomics, respectively. Exercise transiently increased cytokine and chemokine gene expression in synovium-IFP tissue, resolving within the first 5 days of exercise. The acute inflammatory response was associated with decreased adipocyte size and elevated CD45 + Gr1 + myeloid cells, increased collagen content, and oxidized phospholipids. Exercise acutely altered synovial fluid metabolites, characterized by increased amino acids, peptides, bile acids, sphingolipids, dicarboxylic acids, and straight medium chain fatty acids and decreased hydroxy fatty acids and diacylglycerols. Between 5 and 14 days of exercise, inflammation, collagen, and adipocyte size returned to pre-exercise levels, and CD206 + immuno-regulatory macrophages increased. Thus, although the onset of new daily exercise transiently induced synovium-IFP inflammation and altered tissue structure, sustained daily exercise promoted IFP homeostasis.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Exercise induces dynamic changes in intra-articular metabolism and inflammation associated with remodeling of the infrapatellar fat pad in mice
- Date Crossref
- 18/01/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Veterans Health Administration pays non établi dans la noticeOrganisme public
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Oklahoma Medical Research Foundation Aging and Metabolism Research Program pays non établi dans la noticeOrganisation à but non lucratif
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University of Oklahoma Health Sciences Center Department of Biochemistry and Molecular Biology pays non établi dans la noticeÉtablissement de santé
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University of Oklahoma pays non établi dans la noticeUniversité ou école supérieure
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Montana State University Department of Chemistry & Biochemistry pays non établi dans la noticeUniversité ou école supérieure
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Veterans Affairs Medical Center pays non établi dans la noticeÉtablissement de santé
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Arthritis and Clinical Immunology Research Program pays non établi dans la noticeÉtablissement de santé
Veterans Health Administration, Aging and Metabolism Research Program — Oklahoma Medical Research Foundation et Department of Biochemistry and Molecular Biology — University of Oklahoma Health Sciences Center, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.