Histone deacetylase inhibition enhances extracellular vesicles from muscle to promote osteogenesis via miR-873-3p
Rattachement africain : cn, sg. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Regular physical activity is widely recognized for reducing the risk of various disorders, with skeletal muscles playing a key role by releasing biomolecules that benefit multiple organs and tissues. However, many individuals, particularly the elderly and those with clinical conditions, are unable to engage in physical exercise, necessitating alternative strategies to stimulate muscle cells to secrete beneficial biomolecules. Histone acetylation and deacetylation significantly influence exercise-induced gene expression, suggesting that targeting histone deacetylases (HDACs) could mimic some exercise responses. In this study, we explored the effects of the HDAC inhibitor Trichostatin A (TSA) on human skeletal muscle myoblasts (HSMMs). Our findings showed that TSA-induced hyperacetylation enhanced myotube fusion and increased the secretion of extracellular vesicles (EVs) enriched with miR-873-3p. These TSA-EVs promoted osteogenic differentiation in human bone marrow mesenchymal stem cells (hBMSCs) by targeting H2 calponin (CNN2). In vivo, systemic administration of TSA-EVs to osteoporosis mice resulted in significant improvements in bone mass. Moreover, TSA-EVs mimicked the osteogenic benefits of exercise-induced EVs, suggesting that HDAC inhibition can replicate exercise-induced bone health benefits. These results demonstrate the potential of TSA-induced muscle-derived EVs as a therapeutic strategy to enhance bone formation and prevent osteoporosis, particularly for individuals unable to exercise. Given the FDA-approved status of various HDAC inhibitors, this approach holds significant promise for rapid clinical translation in osteoporosis treatment.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Histone deacetylase inhibition enhances extracellular vesicles from muscle to promote osteogenesis via miR-873-3p
- Date Crossref
- 30/09/2024
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Chinese PLA General Hospital Senior Department of Orthopedics pays non établi dans la noticeÉtablissement de santé
-
Harbin Medical University The Department of Orthopedic Surgery pays non établi dans la noticeUniversité ou école supérieure
-
Second Affiliated Hospital of Harbin Medical University pays non établi dans la noticeÉtablissement de santé
-
Nanyang Technological University pays non établi dans la noticeUniversité ou école supérieure
-
Nanjing University Jiangsu Key Laboratory of Molecular Medicine pays non établi dans la noticeUniversité ou école supérieure
-
National Clinical Research Center for Digestive Diseases pays non établi dans la noticeÉtablissement de santé
-
National Clinical Research Center for Orthopedics pays non établi dans la noticeStructure de recherche
-
School of Materials Science and Engineering Max Planck-NTU Joint Lab for Artificial Senses pays non établi dans la noticeUniversité ou école supérieure
Senior Department of Orthopedics — Chinese PLA General Hospital, The Department of Orthopedic Surgery — Harbin Medical University et Second Affiliated Hospital of Harbin Medical University, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.