Dynamics of CWD prion detection in feces and blood from naturally infected white-tailed deer
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Le résumé fourni par la source
Chronic wasting disease (CWD) is a prion disease affecting cervids. Confirmatory testing of CWD is currently performed postmortem in obex and lymphoid tissues. Extensive evidence demonstrates the presence of infectious prions in feces of CWD-infected deer using in vitro prion-amplification techniques and bioassays. In experimental conditions, this has been achieved as soon as 6-month post-inoculation, suggesting this sample type is a candidate for antemortem diagnosis. In the present study, we optimized the detection of CWD-prions in fecal samples from naturally infected, pre-clinical white-tailed deer by comparing protocols aiming to concentrate CWD-prions with direct spiking of the sample into the PMCA reactions. Results of this screening were compared with similar analyses made in blood. Our data shows that CWD-prion detection in feces using PMCA is best in the absence of sample pre-treatments. We performed a screening of 169 fecal samples, detecting CWD-prions with diagnostic sensitivity and specificity of 54.81% and 98.46%, respectively. In addition, the PMCA seeding activity of 76 fecal samples was compared with that on blood of matched deer. Our findings, demonstrate that CWD-prions in feces and blood are increased at late pre-clinical stages, exhibiting similar detection in both sample types (> 90% sensitivity) when PrP96GG animals are tested. Our findings contribute to understand prion distribution across different biological samples and polymorphic variants in white-tailed deer. This information is also relevant for the current efforts to identify platforms to diagnose CWD.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Dynamics of CWD prion detection in feces and blood from naturally infected white-tailed deer
- Date Crossref
- 17/11/2023
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universidad Bernardo O'Higgins pays non établi dans la noticeUniversité ou école supérieure
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The University of Texas Health Science Center at Houston Department of Neurology pays non établi dans la noticeUniversité ou école supérieure
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Animal and Plant Health Inspection Service pays non établi dans la noticeOrganisme public
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United States Department of Agriculture pays non établi dans la noticeOrganisme public
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The University of Texas Health Science Center pays non établi dans la noticeUniversité ou école supérieure
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Centro Integrativo de Biologia y Quimica Aplicada (CIBQA) pays non établi dans la noticeInstitution
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Veterinary Services Cervid Health Program pays non établi dans la noticeInstitution
Universidad Bernardo O'Higgins, Department of Neurology — The University of Texas Health Science Center at Houston et Animal and Plant Health Inspection Service, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.