On the reproducibility of extrusion-based bioprinting: round robin study on standardization in the field
Rattachement africain : de, cz, us, ua, cn. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
The outcome of three-dimensional (3D) bioprinting heavily depends, amongst others, on the interaction between the developed bioink, the printing process, and the printing equipment. However, if this interplay is ensured, bioprinting promises unmatched possibilities in the health care area. To pave the way for comparing newly developed biomaterials, clinical studies, and medical applications (i.e. printed organs, patient-specific tissues), there is a great need for standardization of manufacturing methods in order to enable technology transfers. Despite the importance of such standardization, there is currently a tremendous lack of empirical data that examines the reproducibility and robustness of production in more than one location at a time. In this work, we present data derived from a round robin test for extrusion-based 3D printing performance comprising 12 different academic laboratories throughout Germany and analyze the respective prints using automated image analysis (IA) in three independent academic groups. The fabrication of objects from polymer solutions was standardized as much as currently possible to allow studying the comparability of results from different laboratories. This study has led to the conclusion that current standardization conditions still leave room for the intervention of operators due to missing automation of the equipment. This affects significantly the reproducibility and comparability of bioprinting experiments in multiple laboratories. Nevertheless, automated IA proved to be a suitable methodology for quality assurance as three independently developed workflows achieved similar results. Moreover, the extracted data describing geometric features showed how the function of printers affects the quality of the printed object. A significant step toward standardization of the process was made as an infrastructure for distribution of material and methods, as well as for data transfer and storage was successfully established.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- On the reproducibility of extrusion-based bioprinting: round robin study on standardization in the field
- Date Crossref
- 11/10/2023
- Éditeur
- IOP Publishing
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Karlsruhe Institute of Technology pays non établi dans la noticeUniversité ou école supérieure
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FIZ Karlsruhe – Leibniz Institute for Information Infrastructure pays non établi dans la noticeOrganisation à but non lucratif
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University of Würzburg Department for Functional Materials in Medicine and Dentistry pays non établi dans la noticeUniversité ou école supérieure
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Bavarian Polymer Institute pays non établi dans la noticeStructure de recherche
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University of Bayreuth Bavarian Polymer Institute pays non établi dans la noticeUniversité ou école supérieure
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Natural and Medical Sciences Institute pays non établi dans la noticeInstitution
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University of Tübingen Institute of Biomedical Engineering pays non établi dans la noticeUniversité ou école supérieure
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Medical Technologies (Czechia) pays non établi dans la noticeEntreprise
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University of Freiburg Laboratory for MEMS Applications pays non établi dans la noticeUniversité ou école supérieure
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University of Stuttgart Institute of Interfacial Process Engineering and Plasma Technology pays non établi dans la noticeUniversité ou école supérieure
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Fraunhofer Institute for Interfacial Engineering and Biotechnology Functional Surfaces and Materials pays non établi dans la noticeStructure de recherche
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Plasma Technology (United States) pays non établi dans la noticeEntreprise
Karlsruhe Institute of Technology, FIZ Karlsruhe – Leibniz Institute for Information Infrastructure et Department for Functional Materials in Medicine and Dentistry — University of Würzburg, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.