Transcriptomic profiling reveals differential cellular response to copper oxide nanoparticles and polystyrene nanoplastics in perfused human placenta
Rattachement africain : ch, fi, lu. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
The growing nanoparticulate pollution (e.g. engineered nanoparticles (NPs) or nanoplastics) has been shown to pose potential threats to human health. In particular, sensitive populations such as pregnant women and their unborn children need to be protected from harmful environmental exposures. However, developmental toxicity from prenatal exposure to pollution particles is not yet well studied despite evidence of particle accumulation in human placenta. Our study aimed to investigate how copper oxide NPs (CuO NPs; 10-20 nm) and polystyrene nanoplastics (PS NPs; 70 nm) impact on gene expression in ex vivo perfused human placental tissue. Whole genome microarray analysis revealed changes in global gene expression profile after 6 h of perfusion with sub-cytotoxic concentrations of CuO (10 µg/mL) and PS NPs (25 µg/mL). Pathway and gene ontology enrichment analysis of the differentially expressed genes suggested that CuO and PS NPs trigger distinct cellular response in placental tissue. While CuO NPs induced pathways related to angiogenesis, protein misfolding and heat shock responses, PS NPs affected the expression of genes related to inflammation and iron homeostasis. The observed effects on protein misfolding, cytokine signaling, and hormones were corroborated by western blot (accumulation of polyubiquitinated proteins) or qPCR analysis. Overall, the results of the present study revealed extensive and material-specific interference of CuO and PS NPs with placental gene expression from a single short-term exposure which deserves increasing attention. In addition, the placenta, which is often neglected in developmental toxicity studies, should be a key focus in the future safety assessment of NPs in pregnancy.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Transcriptomic profiling reveals differential cellular response to copper oxide nanoparticles and polystyrene nanoplastics in perfused human placenta
- Date Crossref
- 01/07/2023
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Swiss Federal Laboratories for Materials Science and Technology pays non établi dans la noticeStructure de recherche
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Tampere University pays non établi dans la noticeUniversité ou école supérieure
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Luxembourg Institute of Health pays non établi dans la noticeOrganisation à but non lucratif
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University of Eastern Finland pays non établi dans la noticeUniversité ou école supérieure
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University of Helsinki Institute of Biotechnology pays non établi dans la noticeUniversité ou école supérieure
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Tampere University of Applied Sciences pays non établi dans la noticeUniversité ou école supérieure
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Laboratory for Particles-Biology Interactions pays non établi dans la noticeStructure de recherche
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Faculty of Medicine and Health Technology Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE) pays non établi dans la noticeUniversité ou école supérieure
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Human Biomonitoring Research Unit pays non établi dans la noticeStructure de recherche
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Laboratory for Advanced Analytical Technologies pays non établi dans la noticeStructure de recherche
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School of Medicine Institute of Biomedicine pays non établi dans la noticeUniversité ou école supérieure
Swiss Federal Laboratories for Materials Science and Technology, Tampere University et Luxembourg Institute of Health, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.