P070 Antimicrobial peptides from the Coleoptera family Scarabaeidae against Candida and Cryptococcus pathogenic yeasts.
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Le résumé fourni par la source
Abstract Poster session 1, September 21, 2022, 12:30 PM - 1:30 PM Objectives Host defense peptides (HDP) are produced by a diversity of beetles. The aims of this work were (1) to find new promising peptides from the Coleoptera family Scarabaeidae with potential biomedical applications, (2) to modify physicochemical and structural characteristics of one of the most promissory peptides in order to improve its antimicrobial properties, and (3) to evaluate the in vitro activity of the HDPs against reference strains of pathogenic Candida and Cryptococcus yeasts. Materials and Methods From the Scarabaeidae family transcriptome, 14 promising HDPs were identified. Subsequently, we designed 19 new sequences from Act8 peptide modifying the net charge, hydrophobic angle, and the general composition of amino acids, among other properties, in order to improve the HDPs antifungal activity. The in vitro antifungal susceptibility of the 33 HDPs against C. albicans SC5314, C. krusei, ATCC 6558, C. parapsilosis ATCC 22019, C. glabrata ATCC 2001, C. tropicalis ATCC 750, C. neoformans H99, and C. gattii H0058-I-2029 isolates were evaluated by broth microdilution, with a concentration ranging from 0.19 to 50 μg/ml. Results All 14 peptides identified showed in vitro activity against C. krusei, C. parapsilosis, and C. glabrata. One peptide showed in vitro activity against C. albicans, 6 against C. tropicalis, 11 against C. neoformans and 13 against C. gattii. As well the 19 modified peptides showed in vitro activity against C. krusei, C. parapsilosis, C. tropicalis, C. neoformans, and C. gattii. A total of 15 modified peptides showed in vitro activity against C. albicans, and 3 against C. glabrata. MIC ranges per species and per peptide are shown in Table 1. Conclusions The HDPs herein analyzed showed a significant in vitro antifungal activity against six Candida and two Cryptococcus pathogenic species. Our findings encourage further work with in vivo experimental models in order to better understand the action mechanisms of these antimicrobial peptides. HDPs from different species are becoming a promising therapeutic alternative in the control of fungal infections.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- P070 Antimicrobial peptides from the <i>Coleoptera</i> family <i>Scarabaeidae</i> against <i>Candida</i> and <i>Cryptococcus</i> pathogenic yeasts.
- Date Crossref
- 01/09/2022
- Éditeur
- Oxford University Press (OUP)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Quindío pays non établi dans la noticeUniversité ou école supérieure
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Universidad del Rosario pays non établi dans la noticeUniversité ou école supérieure
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Universitat Pompeu Fabra pays non établi dans la noticeUniversité ou école supérieure
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Mexican Social Security Institute pays non établi dans la noticeOrganisme public
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Universidad del Quindío pays non établi dans la noticeInstitution
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School of Medicine and Health Sciences Studies in Translational Microbiology and Emerging Diseases Research Group (MICROS) pays non établi dans la noticeUniversité ou école supérieure
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Pompeu Fabra University pays non établi dans la noticeUniversité ou école supérieure
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Instituto Mexicano del Seguro Social pays non établi dans la noticeStructure de recherche
University of Quindío, Universidad del Rosario et Universitat Pompeu Fabra, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.