P002 Antifungal activity of antimicrobial synthetic peptides against Candida species of public health importance
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Le résumé fourni par la source
Abstract Poster session 1, September 21, 2022, 12:30 PM - 1:30 PM Background Candidiasis is one of the most frequent opportunistic infections in immunosuppressed and/or hospitalized patients. In countries like Colombia, candidiasis is associated with a mortality rate of ∼ 46%. Growing pharmacological resistance of Candida spp., and the appearance of the emerging pathogen Candida auris, have turned candidiasis into a major public health problem. Different types of antimicrobial peptides have been investigated as a therapeutic alternative to control candidiasis effectively and safely. Objective This work aimed at evaluating the in vitro antifungal activity of three synthetic antimicrobial peptides (35 409, 1609, and 29 009) obtained from Plasmodium falciparum Rif1 protein against C. auris, C. albicans, C. glabrata, C. parapsilosis, C. krusei, and C. tropicalis, species with worldwide clinical importance. Methods The minimum inhibitory concentrations (MIC) of the three peptides against Candida species were determined by the plate microdilution method; the peptides’ effect on biofilm formation in C. auris and C. albicans species was also evaluated through the XTT metabolic activity assay. Additionally, the structural damages in C. auris and C. albicans caused by the action of the peptides were observed by transmission electron microscopy (TEM) and finally, the in vitro peptides’ cytotoxicity against L929 murine fibroblasts was verified. Results Our findings showed that the three peptides herein evaluated, displayed antifungal activity in both planktonic and sessile Candida cells. Likewise, the TEM evidenced morphological alterations induced by the peptides, both in the membrane and at the intracellular level of the yeasts. As well, total safety against the murine cell line L929 with 24 h of treatment was observed. Conclusions From these results, we conclude that the antimicrobial peptides 35 409, 1609, and 29 009 are potential therapeutic alternatives against the most important Candida species in Colombia and the world.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- P002 Antifungal activity of antimicrobial synthetic peptides against <i>Candida</i> species of public health importance
- Date Crossref
- 01/09/2022
- Éditeur
- Oxford University Press (OUP)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universidad del Rosario pays non établi dans la noticeUniversité ou école supérieure
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Fundación Instituto de Inmunología de Colombia Receptor-Ligand Department pays non établi dans la noticeOrganisation à but non lucratif
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Universidad Santo Tomás pays non établi dans la noticeUniversité ou école supérieure
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Universidad Nacional de Colombia pays non établi dans la noticeUniversité ou école supérieure
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School of Medicine and Health Sciences Studies in Translational Microbiology and Emerging Diseases Research Group (MICROS) pays non établi dans la noticeUniversité ou école supérieure
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National Institute of Health Group of Microbiology pays non établi dans la noticeStructure de recherche
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Health Sciences Division pays non établi dans la noticeInstitution
Universidad del Rosario, Receptor-Ligand Department — Fundación Instituto de Inmunología de Colombia et Universidad Santo Tomás, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.