Autoantibodies against Complement Classical Pathway Components C1q, C1r, C1s and C1-Inh in Patients with Lupus Nephritis
Rattachement africain : bg, gb, fr. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Autoantibodies against the complement component C1q (anti-C1q) are among the main biomarkers in lupus nephritis (LN) known to contribute to renal injury. C1q, the recognition subcomponent of the complement classical pathway, forms a heterotetrameric complex with C1r and C1s, and can also associate a central complement regulator and C1 Inhibitor (C1-Inh). However, the frequency and the pathogenic relevance of anti-C1r, anti-C1s and anti-C1-Inh autoantibodies remain poorly studied in LN. In this paper, we screened for anti-C1q, anti-C1r, anti-C1s and anti-C1-Inh autoantibodies and evaluated their association with disease activity and severity in 74 LN patients followed up for 5 years with a total of 266 plasma samples collected. The presence of anti-C1q, anti-C1r, anti-C1s and anti-C1-Inh was assessed by ELISA. IgG was purified by Protein G from antigen-positive plasma and their binding to purified C1q, C1r and C1s was examined by surface plasmon resonance (SPR). The abilities of anti-C1q, anti-C1r and anti-C1s binding IgG on C1 complex formation were analyzed by ELISA. The screening of LN patients' plasma revealed 14.9% anti-C1q positivity; only 4.2%, 6.9% and 0% were found to be positive for anti-C1r, anti-C1s and anti-C1-Inh, respectively. Significant correlations were found between anti-C1q and anti-dsDNA, and anti-nuclear antibodies, C3 and C4, respectively. High levels of anti-C1q antibodies were significantly associated with renal histologic lesions and correlated with histological activity index. Patients with the most severe disease (A class according to BILAG Renal score) had higher levels of anti-C1q antibodies. Anti-C1r and anti-C1s antibodies did not correlate with the clinical characteristics of the LN patients, did not interfere with the C1 complex formation, and were not measurable via SPR. In conclusion, the presence of anti-C1q, but not anti-C1s or anti-C1r, autoantibodies contribute to the autoimmune pathology and the severity of LN.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Autoantibodies against Complement Classical Pathway Components C1q, C1r, C1s and C1-Inh in Patients with Lupus Nephritis
- Date Crossref
- 17/08/2022
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Medical University of Varna Department of Biochemistry pays non établi dans la noticeUniversité ou école supérieure
-
Queen Giovanna Hospital pays non établi dans la noticeÉtablissement de santé
-
Medical University of Sofia Department of Nephrology pays non établi dans la noticeUniversité ou école supérieure
-
Brunel University of London pays non établi dans la noticeUniversité ou école supérieure
-
Inserm pays non établi dans la noticeOrganisme public
-
Université Paris Cité pays non établi dans la noticeUniversité ou école supérieure
-
Sorbonne Université Centre de Recherche des Cordeliers pays non établi dans la noticeUniversité ou école supérieure
-
Centre de Recherche des Cordeliers pays non établi dans la noticeStructure de recherche
-
University Hospital “Tsaritza Yoanna—ISUL” Clinic of Nephrology pays non établi dans la noticeUniversité ou école supérieure
-
College of Health Department of Life Sciences pays non établi dans la noticeUniversité ou école supérieure
-
U.A.E. University Department of Veterinary Medicine pays non établi dans la noticeUniversité ou école supérieure
Department of Biochemistry — Medical University of Varna, Queen Giovanna Hospital et Department of Nephrology — Medical University of Sofia, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.