Evaluation of Intracellular Processes in Quinolinic Acid-Induced Brain Damage by Imaging Reactive Oxygen Species Generation and Mitochondrial Complex I Activity
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Le résumé fourni par la source
Abstract Purpose Our study aimed to elucidate the intracellular processes associated with quinolinic acid (QA)-induced brain injury by acquiring semiquantitative fluorescent images of reactive oxygen species (ROS) generation and positron emission tomography (PET) images of mitochondrial complex I (MC-I) activity.MethodsEx vivo fluorescent imaging with dihydroethidium (DHE) and PET scans with 18F-BCPP-EF were conducted at 3 hours and 1 day after QA injection into the rat striatum. Immunohistochemical studies were performed 1 day after QA injection into the rat brain using monoclonal antibodies against neuronal nuclei (NeuN) and CD11b.ResultsA strong DHE-derived fluorescent signal was detected in a focal area within the QA-injected striatum 3 hours after QA injection, and increased fluorescent signal spread throughout the striatum and parts of the cerebral cortex after 1 day. By contrast, 18F-BCPP-EF uptake in the QA-injected rat brain was unchanged after 3 hours and markedly decreased after 1 day, not only in the striatum but also in the cerebral hemisphere. The fluorescent signal in the striatum 1 day after QA injection colocalised with microglial marker expression.ConclusionsWe successfully obtained functional images of focal ROS generation during the early period of excitotoxic injury, and microglial ROS generation and mitochondrial dysfunction were observed during the progression of the inflammatory response. Both ex vivo DHE imaging and in vivo 18F-BCPP-EF-PET were sufficiently sensitive to detect the respective processes of QA-induced brain damage. Our study contributes to the functional imaging of multiple events during the pathological process.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Evaluation of Intracellular Processes in Quinolinic Acid-Induced Brain Damage by Imaging Reactive Oxygen Species Generation and Mitochondrial Complex I Activity
- Date Crossref
- 10/05/2021
- Éditeur
- Springer Science and Business Media LLC
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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The University of Osaka pays non établi dans la noticeUniversité ou école supérieure
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Hamamatsu Photonics (United Kingdom) pays non établi dans la noticeEntreprise
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Showa Pharmaceutical University pays non établi dans la noticeUniversité ou école supérieure
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University of Shizuoka pays non établi dans la noticeUniversité ou école supérieure
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Chiba University pays non établi dans la noticeUniversité ou école supérieure
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Osaka University pays non établi dans la noticeUniversité ou école supérieure
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Hamamatsu Photonics K.K pays non établi dans la noticeInstitution
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Hanwa Intelligent Medical Center pays non établi dans la noticeÉtablissement de santé
The University of Osaka, Hamamatsu Photonics (United Kingdom) et Showa Pharmaceutical University, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.