Gene signature fingerprints stratify SLE patients in groups with similar biological disease profiles: a multicentre longitudinal study
Rattachement africain : nl, cz. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
OBJECTIVES: Clinical phenotyping and predicting treatment responses in SLE patients is challenging. Extensive blood transcriptional profiling has identified various gene modules that are promising for stratification of SLE patients. We aimed to translate existing transcriptomic data into simpler gene signatures suitable for daily clinical practice. METHODS: Real-time PCR of multiple genes from the IFN M1.2, IFN M5.12, neutrophil (NPh) and plasma cell (PLC) modules, followed by a principle component analysis, was used to identify indicator genes per gene signature. Gene signatures were measured in longitudinal samples from two childhood-onset SLE cohorts (n = 101 and n = 34, respectively), and associations with clinical features were assessed. Disease activity was measured using Safety of Estrogen in Lupus National Assessment (SELENA)-SLEDAI. Cluster analysis subdivided patients into three mutually exclusive fingerprint-groups termed (1) all-signatures-low, (2) only IFN high (M1.2 and/or M5.12) and (3) high NPh and/or PLC. RESULTS: All gene signatures were significantly associated with disease activity in cross-sectionally collected samples. The PLC-signature showed the highest association with disease activity. Interestingly, in longitudinally collected samples, the PLC-signature was associated with disease activity and showed a decrease over time. When patients were divided into fingerprints, the highest disease activity was observed in the high NPh and/or PLC group. The lowest disease activity was observed in the all-signatures-low group. The same distribution was reproduced in samples from an independent SLE cohort. CONCLUSIONS: The identified gene signatures were associated with disease activity and were indicated to be suitable tools for stratifying SLE patients into groups with similar activated immune pathways that may guide future treatment choices.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Gene signature fingerprints stratify SLE patients in groups with similar biological disease profiles: a multicentre longitudinal study
- Date Crossref
- 10/02/2022
- Éditeur
- Oxford University Press (OUP)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Erasmus MC pays non établi dans la noticeÉtablissement de santé
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Emma Kinderziekenhuis pays non établi dans la noticeÉtablissement de santé
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University of Amsterdam pays non établi dans la noticeUniversité ou école supérieure
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Amalia Kinderziekenhuis pays non établi dans la noticeÉtablissement de santé
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Sint Maartenskliniek pays non établi dans la noticeÉtablissement de santé
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Leiden University Medical Center Department of Pediatrics pays non établi dans la noticeOrganisme public
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University Hospital Olomouc pays non établi dans la noticeÉtablissement de santé
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Palacký University Olomouc pays non établi dans la noticeUniversité ou école supérieure
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Department of Immunology pays non établi dans la noticeInstitution
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University Medical Center Rotterdam Department of Paediatric Rheumatology pays non établi dans la noticeUniversité ou école supérieure
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Amsterdam University Medical Centres Department of Pediatric Immunology pays non établi dans la noticeUniversité ou école supérieure
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Amalia Children’s Hospital Department of Paediatric Rheumatology pays non établi dans la noticeÉtablissement de santé
Erasmus MC, Emma Kinderziekenhuis et University of Amsterdam, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.