Prolonged storage of purified granulocyte concentrates: Introduction of a new purification method
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Le résumé fourni par la source
Abstract Background Use of donor granulocyte concentrate (GC) has been limited due to its short storage time of 6–24 h, which is partially due to residual red blood cells (RBCs) and platelets and the resulting lactate production leading to an acidotic milieu. To increase this storage time, we developed a closed system procedure compatible with standard blood bank technologies to remove RBC and platelets and to enrich the GC. Methods Standard GCs (sGCs) were sedimented, washed twice with 0.9% sodium chloride (NaCl), and resuspended in blood group‐identical fresh frozen plasma. The resulting purified GCs (pGCs) were then stored in platelet bags at a cell concentration of about 5 × 107 ± 1.8 × 107 leukocytes/ml without agitation at room temperature for up to 72 h. Cell count and viability, pH, blood gases, phagocytosis, and oxidative burst were monitored daily. Results A significant reduction in RBC (98%) through sedimentation, and platelets (96%) by washing, purified the white blood cell (WBC) population and enriched the granulocytes to 96% of the WBC in the pGC. After 72 h of storage, over 90% of the initial WBC count of pGC remained, was viable (≥97%), and the granulocytes exhibited a high phagocytosis and oxidative burst functionality, comparable to sGC after 24 h. Conclusion Purification extends the maximum storage period of GC from 24 to 72 h and may therefore improve the availability of GC and its clinical use.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Prolonged storage of purified granulocyte concentrates: Introduction of a new purification method
- Date Crossref
- 16/11/2021
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Rostock Department of Anaesthesiology and Intensive Care Medicine pays non établi dans la noticeUniversité ou école supérieure
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Medizinische Hochschule Hannover pays non établi dans la noticeUniversité ou école supérieure
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Fraunhofer Institute for Cell Therapy and Immunology Department of Extracorporeal Immunomodulation pays non établi dans la noticeStructure de recherche
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Artcline GmbH Rostock Germany pays non établi dans la noticeEntreprise
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Institute of Transfusion Medicine and Transplant Engineering Hannover Medical School Hannover Germany pays non établi dans la noticeStructure de recherche
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Medical Faculty Department of Medicine pays non établi dans la noticeUniversité ou école supérieure
Department of Anaesthesiology and Intensive Care Medicine — University of Rostock, Medizinische Hochschule Hannover et Department of Extracorporeal Immunomodulation — Fraunhofer Institute for Cell Therapy and Immunology, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.