The Kg‐antigen, RhAG with a Lys164Gln mutation, gives rise to haemolytic disease of the newborn
Rattachement africain : jp. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
The Kg-antigen was first discovered in an investigation of a mother whose infant had haemolytic disease of the newborn (HDN). The antibody against the Kg-antigen is believed to be responsible for HDN. The Kg-antigen is provisionally registered under the number 700045, according to the Red Cell Immunogenetics and Blood Group Terminology. However, the molecular nature of the Kg-antigen has remained a mystery for over 30 years. In this study, a monoclonal antibody against the Kg-antigen and the recombinant protein were developed that allowed for the immunoprecipitation analysis. Immunoprecipitants from the propositus' red blood cell ghosts were subjected to mass spectrometry analysis, and DNA sequence analysis of the genes was also performed. A candidate for the Kg-antigen was molecularly isolated and confirmed to be a determinant of the Kg-antigen by cell transfection and flow cytometry analyses. The Kg-antigen and the genetic mutation were then screened for in a Japanese population. The molecular nature of the Kg-antigen was shown to be RhAG with a Lys164Gln mutation. Kg phenotyping further clarified that 0.22% of the Japanese population studied was positive for the Kg-antigen. These findings provide important information on the Kg-antigen, which has been clinically presumed to give rise to HDN.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- The Kg‐antigen, RhAG with a Lys164Gln mutation, gives rise to haemolytic disease of the newborn
- Date Crossref
- 23/07/2020
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Japanese Red Cross Society pays non établi dans la noticeOrganisation à but non lucratif
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National Institute of Biomedical Innovation pays non établi dans la noticeStructure de recherche
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Japanese Red Cross Kinki Block Blood Center Osaka Japan pays non établi dans la noticeInstitution
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Japanese Red Cross Central Blood Institute Tokyo Japan pays non établi dans la noticeStructure de recherche
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National Institutes of Biomedical Innovation pays non établi dans la noticeStructure de recherche
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Japanese Red Cross Kanto‐koshinetsu Block Blood Center Tokyo Japan pays non établi dans la noticeInstitution
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Japanese Red Cross Kanto-koshinetsu Block Blood Center pays non établi dans la noticeInstitution
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Tsukiyama Child Care Clinic Wakayama Japan pays non établi dans la noticeÉtablissement de santé
Japanese Red Cross Society, National Institute of Biomedical Innovation et Japanese Red Cross Kinki Block Blood Center Osaka Japan, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.