Comprehensive analysis of soluble RNAs in human embryo culture media and blastocoel fluid
Rattachement africain : dk, se. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
PURPOSE: miRNAs have been suggested as biomarkers of embryo viability; however, findings from preliminary studies are divergent. Furthermore, the presence of other types of small RNA molecules remains to be investigated. The purpose of this study was to perform a comprehensive analysis of small non-coding RNA levels in spent and unconditioned embryo culture media, along with miRNA levels in blastocoelic fluid samples from human embryos. METHODS: miRNAs in unconditioned culture medium from 3 different manufacturers, along with miRNA from day 5 conditioned culture medium, control medium, and corresponding blastocoel fluid from 10 human blastocysts were analyzed with array-based q-PCR analysis. Subsequently, deep sequencing of total and small RNA in day 5 spent culture medium from 5 human blastocysts and corresponding controls was performed. RESULTS: values were above the recommended limit for detection in the array-based analysis, a finding that was confirmed by deep sequencing. The majority of miRNAs identified by deep sequencing were expressed in all samples including control media and seem to originate from sources other than conditioned IVF media. CONCLUSIONS: Our findings question the use of miRNAs as a reliable biomarker and highlight the need for a critical methodological approach in miRNA studies. Interestingly, tiRNA fragments appear to be overexpressed in conditioned IVF media samples and could potentially be a novel biomarker worthy of investigation.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Comprehensive analysis of soluble RNAs in human embryo culture media and blastocoel fluid
- Date Crossref
- 17/07/2020
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Aarhus University Hospital Department of Clinical Biochemistry pays non établi dans la noticeÉtablissement de santé
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Aarhus University pays non établi dans la noticeUniversité ou école supérieure
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Fertilitetscentrum pays non établi dans la noticeÉtablissement de santé
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Sahlgrenska University Hospital Unit of Reproductive Medicine pays non établi dans la noticeÉtablissement de santé
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Aalborg University Hospital Fertility Unit and Centre for Preimplantation diagnosis pays non établi dans la noticeÉtablissement de santé
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Regional Hospital Horsens pays non établi dans la noticeÉtablissement de santé
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Fertility Clinic pays non établi dans la noticeÉtablissement de santé
Department of Clinical Biochemistry — Aarhus University Hospital, Aarhus University et Fertilitetscentrum, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.