Arm movements induced by noninvasive optogenetic stimulation of the motor cortex in the common marmoset
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Le résumé fourni par la source
Optogenetics is now a fundamental tool for investigating the relationship between neuronal activity and behavior. However, its application to the investigation of motor control systems in nonhuman primates is rather limited, because optogenetic stimulation of cortical neurons in nonhuman primates has failed to induce or modulate any hand/arm movements. Here, we used a tetracycline-inducible gene expression system carrying CaMKII promoter and the gene encoding a Channelrhodopsin-2 variant with fast kinetics in the common marmoset, a small New World monkey. In an awake state, forelimb movements could be induced when Channelrhodopsin-2-expressing neurons in the motor cortex were illuminated by blue laser light with a spot diameter of 1 mm or 2 mm through a cranial window without cortical invasion. Forelimb muscles responded 10 ms to 50 ms after photostimulation onset. Long-duration (500 ms) photostimulation induced discrete forelimb movements that could be markerlessly tracked with charge-coupled device cameras and a deep learning algorithm. Long-duration photostimulation mapping revealed that the primary motor cortex is divided into multiple domains that can induce hand and elbow movements in different directions. During performance of a forelimb movement task, movement trajectories were modulated by weak photostimulation, which did not induce visible forelimb movements at rest, around the onset of task-relevant movement. The modulation was biased toward the movement direction induced by the strong photostimulation. Combined with calcium imaging, all-optical interrogation of motor circuits should be possible in behaving marmosets.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Arm movements induced by noninvasive optogenetic stimulation of the motor cortex in the common marmoset
- Date Crossref
- 21/10/2019
- Éditeur
- National Academy of Sciences
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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The University of Tokyo International Research Center for Neurointelligence pays non établi dans la noticeUniversité ou école supérieure
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RIKEN Center for Brain Science pays non établi dans la noticeStructure de recherche
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Japan Science and Technology Agency pays non établi dans la noticeOrganisme public
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The Graduate University for Advanced Studies pays non établi dans la noticeUniversité ou école supérieure
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National Institute for Physiological Sciences pays non établi dans la noticeStructure de recherche
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Jichi Medical University Center for Molecular Medicine pays non établi dans la noticeUniversité ou école supérieure
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Graduate School of Medicine Department of Physiology pays non établi dans la noticeUniversité ou école supérieure
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Laboratory for Molecular Analysis of Higher Brain Function pays non établi dans la noticeStructure de recherche
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Brain Functional Dynamics Collaboration Laboratory pays non établi dans la noticeStructure de recherche
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School of Life Science National Institute for Physiological Sciences pays non établi dans la noticeUniversité ou école supérieure
International Research Center for Neurointelligence — The University of Tokyo, RIKEN Center for Brain Science et Japan Science and Technology Agency, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.