Non‑canonical Wnt signaling contributes to ventilator‑induced lung injury through upregulation of WISP1 expression
Rattachement africain : cn, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Mechanical ventilation may cause ventilator‑induced lung injury (VILI). Canonical Wnt signaling has been reported to serve an important role in the pathogenesis of VILI. Bioinformatics analysis revealed that canonical and non‑canonical Wnt signaling pathways were activated in VILI. However, the role of non‑canonical Wnt signaling in the pathogenesis of VILI remains unclear. The present study aimed to analyze the potential role of non‑canonical Wnt signaling in VILI pathogenesis. Lung injury was assessed via Evans blue albumin permeability and histological scoring, as well as by inflammatory cytokine expression and total protein concentration in bronchoalveolar lavage fluid. The relative protein expression of canonical and non‑canonical Wnt signaling pathway components were examined via western blotting and immunohistochemistry. The results demonstrated that 6 h of mechanical ventilation at low tidal volume (LTV; 6 ml/kg) or moderate tidal volume (MTV; 12 ml/kg) induced lung injury in sensitive A/J mice. Ventilation with MTV increased the protein levels of Wnt‑induced secreted protein 1 (WISP1), Rho‑associated protein kinase 1 (ROCK1), phosphorylated (p)‑Ras homolog gene family, member A and p‑C‑Jun N‑terminal kinase (JNK). Inhibition of ROCK1 by Y27632 and JNK by SP600125 attenuated MTV‑induced lung injury and decreased the expression of proteins involved in non‑canonical Wnt signaling, including WISP1. In conclusion, non‑canonical Wnt signaling participates in VILI by modulating WISP1 expression, which has been previously noted as critical for VILI development. Therefore, the non‑canonical Wnt signaling pathway may provide a preventive and therapeutic target in VILI.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Non‑canonical Wnt signaling contributes to ventilator‑induced lung injury through upregulation of WISP1 expression
- Date Crossref
- 17/01/2019
- Éditeur
- Spandidos Publications
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Central South University Department of Anesthesiology pays non établi dans la noticeUniversité ou école supérieure
-
Third Xiangya Hospital pays non établi dans la noticeÉtablissement de santé
-
Shanghai Jiao Tong University pays non établi dans la noticeUniversité ou école supérieure
-
Shanghai Children's Medical Center pays non établi dans la noticeÉtablissement de santé
-
Hunan Cancer Hospital Department of Anesthesiology pays non établi dans la noticeÉtablissement de santé
-
University of Pittsburgh Department of Environmental and Occupational Health pays non établi dans la noticeUniversité ou école supérieure
-
Shanghai Jiao‑Tong University School of Medicine Department of Anesthesiology pays non établi dans la noticeUniversité ou école supérieure
Department of Anesthesiology — Central South University, Third Xiangya Hospital et Shanghai Jiao Tong University, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.