Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro
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Le résumé fourni par la source
PURPOSE: The aim of this study was to investigate whether cells originating from human embryonic stem cells (hESCs) could be successfully transplanted onto a partially wounded human cornea. A second aim was to study the ability of the transplanted cells to differentiate into corneal epithelial-like cells. METHODS: Spontaneously, differentiated hESCs were transplanted onto a human corneal button (without limbus) with the epithelial layer partially removed. The cells were cultured on Bowman's membrane for up to 9 days, and the culture dynamics documented in a time-lapse system. As the transplanted cells originated from a genetically engineered hESC line, they all expressed green fluorescent protein, which facilitated their identification during the culture experiments, tissue preparation and analysis. To detect any differentiation into human corneal epithelial-like cells, we analysed the transplanted cells by immunohistochemistry using antibodies specific for CK3, CK15 and PAX6. RESULTS: The transplanted cells established and expanded on Bowman's membrane, forming a 1-4 cell layer surrounded by host corneal epithelial cells. Expression of the corneal marker PAX6 appeared 3 days after transplantation, and after 6 days, the cells were expressing both PAX6 and CK3. CONCLUSION: This shows that it is possible to transplant cells originating from hESCs onto Bowman's membrane with the epithelial layer partially removed and to get these cells to establish, grow and differentiate into corneal epithelial-like cells in vitro.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Transplantation of human embryonic stem cells onto a partially wounded human cornea <i>in vitro</i>
- Date Crossref
- 26/01/2012
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Sahlgrenska University Hospital Unit of Clinical Sciences pays non établi dans la noticeÉtablissement de santé
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University of Gothenburg pays non établi dans la noticeUniversité ou école supérieure
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Fertilitetscentrum pays non établi dans la noticeÉtablissement de santé
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Thermo Fisher Scientific (Norway) pays non établi dans la noticeEntreprise
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Carlanderska Hospital Fertilitetscentrum pays non établi dans la noticeÉtablissement de santé
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Cellartis AB pays non établi dans la noticeInstitution
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Life Technologies pays non établi dans la noticeInstitution
Unit of Clinical Sciences — Sahlgrenska University Hospital, University of Gothenburg et Fertilitetscentrum, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.