Clostridium perfringens α-toxin interaction with red cells and model membranes
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Le résumé fourni par la source
The effects of Clostridium perfringensα-toxin on host cells have previously been studied extensively but the biophysical processes associated with toxicity are poorly understood. The work reported here shows that the initial interaction between the toxin and lipid membrane leads to measurable changes in the physical properties and morphology of the membrane. A Langmuir monolayer technique was used to assess the response of different lipid species to toxin. Sphingomyelin and unsaturated phosphatidylcholine showed the highest susceptibility to toxin lypolitic action, with a two stage response to the toxin (an initial, rapid hydrolysis stage followed by the insertion and/or reorganisation of material in the monolayer). Fluorescence confocal microscopy on unsaturated phosphatidylcholine vesicles shows that the toxin initially aggregates at discrete sites followed by the formation of localised "droplets" accumulating the hydrolysis products. This process is accompanied by local increases in the membrane dipole potential by about 50 (±42) mV. In contrast, red blood cells incubated with the toxin suffered a decrease of the membrane dipole potential by 50 (±40) mV in areas of high toxin activity (equivalent to a change in electric field strength of 10(7) V m(-1)) which is sufficient to affect the functioning of the cell membrane. Changes in erythrocyte morphology caused by the toxin are presented, and the early stages of interaction between toxin and membrane are characterised using thermal shape fluctuation analysis of red cells which revealed two distinct regimes of membrane-toxin interaction.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Clostridium perfringens α-toxin interaction with red cells and model membranes
- Date Crossref
- 01/01/2015
- Éditeur
- Royal Society of Chemistry (RSC)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Exeter pays non établi dans la noticeUniversité ou école supérieure
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University of London Birkbeck pays non établi dans la noticeUniversité ou école supérieure
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School of Physics pays non établi dans la noticeUniversité ou école supérieure
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School of Biosciences pays non établi dans la noticeUniversité ou école supérieure
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Birkbeck College Department of Crystallography pays non établi dans la noticeUniversité ou école supérieure
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Department of Crystallography pays non établi dans la noticeInstitution
University of Exeter, Birkbeck — University of London et School of Physics, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.