Short single-stranded DNA oligonucleotides enable intracellular transcription of functional RNAs in mammalian cells
Ahmed Mahas, Ramiro M. Perrotta, Roman Teo Oliynyk, Raphaël Ferreira et autres
Abstract Programmable intracellular production of short functional RNAs underlies diverse applications ranging from gene regulation to genome engineering, but is often constrained by cloning workflows, RNA synthesis, or viral-vector delivery. Here, we introduce a single-stranded DNA (ssDNA)-driven intracellular transcription strategy in which …
us, dk (code pays fourni par la source)
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bioRxiv (Cold Spring Harbor Laboratory)